Detailed information of TA system TA052340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 73824..74389
• Replicon: chromosome
• Accession: NZ_CP010401
• Organism: Bartonella ancashensis strain 20.00

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: PU02_RS00395
• Protein ID: WP_053943601.1
• Coordinates: 73824..74156 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0M4LHC4
• Locus tag: PU02_RS00400
• Protein ID: WP_053943602.1
• Coordinates: 74153..74389 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PU02_RS00355	69136..69717	-	582	WP_053943594.1	phage tail protein	-
PU02_RS00360	69721..69990	-	270	WP_053943595.1	hypothetical protein	-
PU02_RS00365	70281..70676	-	396	WP_053943596.1	type II toxin-antitoxin system HicB family antitoxin	-
PU02_RS00370	70778..71074	-	297	WP_053943597.1	HigA family addiction module antidote protein	-
PU02_RS00375	71091..71369	-	279	WP_200903957.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PU02_RS00380	71543..71815	-	273	WP_053943567.1	hypothetical protein	-
PU02_RS00385	71812..72552	-	741	WP_053943599.1	antA/AntB antirepressor family protein	-
PU02_RS06630	72806..73297	-	492	WP_053943600.1	Rha family transcriptional regulator	-
PU02_RS06780	73636..73773	-	138	WP_158404006.1	hypothetical protein	-
PU02_RS00395	73824..74156	-	333	WP_053943601.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PU02_RS00400	74153..74389	-	237	WP_053943602.1	antitoxin MazE family protein	Antitoxin
PU02_RS00405	74902..75324	-	423	WP_053943603.1	type II toxin-antitoxin system VapC family toxin	-
PU02_RS06785	75426..75587	-	162	WP_158404007.1	hypothetical protein	-
PU02_RS00410	75666..75872	-	207	WP_144417854.1	hypothetical protein	-
PU02_RS06475	76223..76609	-	387	WP_053944052.1	type II toxin-antitoxin system PemK/MazF family toxin	-
PU02_RS00415	76653..77204	-	552	Protein_82	hypothetical protein	-
PU02_RS00420	77219..77650	-	432	WP_082311381.1	single-stranded DNA-binding protein	-
PU02_RS00425	78309..79329	+	1021	Protein_84	DUF3472 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	36419..85973	49554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin MazE-like (16-76)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11958.06 Da
• Isoelectric Point: 10.6199

>T52340 WP_053943601.1 NZ_CP010401:c74156-73824 [Bartonella ancashensis]
MRRGDLVTVSMQGDFGKPRPALIIQSDIFGKYLASTFTVLPVTSALVSAPLLRITVQPSPENGLQKPSQIMVDKIMTIKH
TKVGSSFGRIDAHTLALVESYLATFLGITR

Nucleotide

• Length: 333 bp

>T52340 NZ_CP010401:c74156-73824 [Bartonella ancashensis]
ATGAGGCGAGGAGATCTTGTGACTGTCTCTATGCAAGGAGATTTCGGAAAACCACGACCTGCTTTAATTATTCAATCTGA
CATATTCGGTAAATACCTTGCTTCTACTTTTACGGTACTTCCAGTAACGAGTGCGCTTGTTTCAGCTCCACTATTACGTA
TTACTGTTCAACCAAGTCCTGAAAATGGTTTGCAAAAGCCATCGCAGATAATGGTAGATAAAATTATGACGATAAAGCAT
ACAAAGGTAGGTTCAAGCTTTGGCCGCATTGATGCTCATACACTTGCGCTTGTGGAAAGCTATTTAGCTACGTTTCTTGG
CATTACCAGGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9307.45 Da
• Isoelectric Point: 4.3757

>AT52340 WP_053943602.1 NZ_CP010401:c74389-74153 [Bartonella ancashensis]
MKHLTAENINLQEFQRNEDALRKVGLHPVQIWVQDTQRPGFEEECRRQCELIKQADMEYGDLQQFMDEALKDIDSWTV

Nucleotide

• Length: 237 bp

>AT52340 NZ_CP010401:c74389-74153 [Bartonella ancashensis]
ATGAAACACCTCACTGCTGAAAATATAAATTTACAGGAATTTCAACGGAATGAAGATGCTCTGCGCAAAGTGGGGTTACA
TCCAGTGCAAATTTGGGTACAAGATACTCAACGACCAGGCTTTGAAGAAGAATGTCGCCGCCAGTGCGAACTTATTAAGC
AAGCAGATATGGAATACGGTGATTTGCAACAATTCATGGATGAGGCGCTAAAAGACATAGACAGTTGGACAGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0M4LHC4