Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36569..37212 Replicon plasmid p34618-71.572kb
Accession NZ_CP010396
Organism Klebsiella pneumoniae strain 34618

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LI86_RS28395 Protein ID WP_001044770.1
Coordinates 36796..37212 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LI86_RS28390 Protein ID WP_001261282.1
Coordinates 36569..36799 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI86_RS28370 34090..34866 - 777 WP_000015958.1 site-specific integrase -
LI86_RS28375 34863..35606 - 744 WP_000129823.1 hypothetical protein -
LI86_RS28380 35657..36007 - 351 WP_000493378.1 hypothetical protein -
LI86_RS28385 36151..36612 - 462 WP_072202616.1 hypothetical protein -
LI86_RS28390 36569..36799 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LI86_RS28395 36796..37212 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LI86_RS28405 37375..38072 + 698 Protein_40 IS1 family transposase -
LI86_RS28410 38073..38198 + 126 WP_072093211.1 mercury transporter -
LI86_RS28415 38234..38656 + 423 WP_001340589.1 organomercurial transporter MerC -
LI86_RS28420 38708..40402 + 1695 WP_000105636.1 mercury(II) reductase -
LI86_RS28425 40420..40782 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
LI86_RS28430 40779..41015 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
LI86_RS28435 41051..41719 + 669 WP_001300294.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ant(3'')-Ia / cmlA1 / aadA2 / aac(6')-Ib / blaTEM-1A / blaOXA-9 / blaKPC-2 / aph(4)-Ia / aac(3)-IVa - 1..71572 71572
- inside IScluster/Tn aac(6')-Ib - 28251..38072 9821


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T52335 WP_001044770.1 NZ_CP010396:36796-37212 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T52335 NZ_CP010396:36796-37212 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT52335 WP_001261282.1 NZ_CP010396:36569-36799 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT52335 NZ_CP010396:36569-36799 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References