Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 41858..42594 Replicon plasmid p34618-207.543kb
Accession NZ_CP010393
Organism Klebsiella pneumoniae strain 34618

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LI86_RS27015 Protein ID WP_003026803.1
Coordinates 41858..42340 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LI86_RS27020 Protein ID WP_003026799.1
Coordinates 42328..42594 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI86_RS26995 37495..38457 + 963 WP_004152113.1 M48 family metalloprotease -
LI86_RS31080 38545..39799 + 1255 Protein_45 IS3 family transposase -
LI86_RS27010 40300..41646 - 1347 WP_020314316.1 ISNCY family transposase -
LI86_RS27015 41858..42340 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LI86_RS27020 42328..42594 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LI86_RS27025 42770..43024 - 255 WP_004152108.1 hypothetical protein -
LI86_RS27030 43100..43357 - 258 WP_004152107.1 hypothetical protein -
LI86_RS27035 43406..43609 - 204 WP_004152106.1 hemolysin expression modulator Hha -
LI86_RS27040 43643..44011 - 369 WP_004152105.1 hypothetical protein -
LI86_RS27045 44055..44549 - 495 WP_004152104.1 DNA-binding protein -
LI86_RS27050 44580..45155 - 576 WP_004152103.1 hypothetical protein -
LI86_RS27055 45143..45412 - 270 WP_004152102.1 hypothetical protein -
LI86_RS27060 45770..46120 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LI86_RS27065 46170..46532 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia - 1..207543 207543


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T52334 WP_003026803.1 NZ_CP010393:c42340-41858 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T52334 NZ_CP010393:c42340-41858 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT52334 WP_003026799.1 NZ_CP010393:c42594-42328 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT52334 NZ_CP010393:c42594-42328 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References