Detailed information of TA system TA052315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 87482..88279
• Replicon: plasmid p34399-121.660kb
• Accession: NZ_CP010386
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain 34399

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: LI66_RS23995
• Protein ID: WP_172754447.1
• Coordinates: 87776..88279 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: LI66_RS23990
• Protein ID: WP_016582626.1
• Coordinates: 87482..87751 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI66_RS23965	82812..84152	-	1341	WP_040110301.1	AAA family ATPase	-
LI66_RS23970	84213..84938	-	726	WP_006812583.1	hypothetical protein	-
LI66_RS23975	85202..85999	+	798	WP_077990606.1	DUF2971 domain-containing protein	-
LI66_RS23980	86041..86394	-	354	WP_160956455.1	hypothetical protein	-
LI66_RS23985	86400..87065	-	666	WP_023315962.1	AAA family ATPase	-
LI66_RS23990	87482..87751	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
LI66_RS23995	87776..88279	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
LI66_RS26070	88307..88513	-	207	WP_080332520.1	hypothetical protein	-
LI66_RS26075	88464..88715	-	252	WP_040110303.1	hypothetical protein	-
LI66_RS24005	88717..89409	-	693	WP_006812500.1	membrane protein	-
LI66_RS24010	89423..89746	-	324	WP_000064173.1	hypothetical protein	-
LI66_RS24015	89821..90609	-	789	WP_040110304.1	receptor-recognizing protein	-
LI66_RS25955	90674..91690	-	1017	Protein_111	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..121660	121660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T52315 WP_172754447.1 NZ_CP010386:87776-88279 [Enterobacter hormaechei subsp. xiangfangensis]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T52315 NZ_CP010386:87776-88279 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTTTTCAGCACAAGCCGTATACGATTTTTCGGGCTTCGACTGCGGCGAACCATCTCTGAATGATTTCCTGCAAAACCG
CCTGGTACAGCAACACAACGGGCGTATATTACGTGGCTACCTGCTTCTTACCAAAGATCCGGTGCCGAAAGTTAAGGGTT
TTTACACGCTATCCGGGAGCTGTTTTGCAAGACAGACGCTACCATCGAACACCCAGAAGCGGAAAATCCCCTACGCGGAT
GCCCCCAGCGTTACGCTTGGCCGTCTGGCAATAGATATCAGCCTACAGAAACAAGGCTACGGGGAGGTTCTCGTAACGCA
CGCGTTGAAGGTTGTTTATCAGGCTTCTCGCGCAGTAGGCATATACGCTTTGTTTGTGGATGCACTAAACCAAAGCGCAA
TGCAGTTCTATCAGAAGTTTGGATTCATCCCTTTGACGGGGGCAAATGCCAACTCGCTTTTCTACCCCACGAAAAGCATT
GAAGAGCTGTTTGAAACAGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT52315 WP_016582626.1 NZ_CP010386:87482-87751 [Enterobacter hormaechei subsp. xiangfangensis]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT52315 NZ_CP010386:87482-87751 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACGATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGCT
TGATTCTCAGTGAAGAGTCCTGGAATCTGGTAATGGATGCCATCAGTAACCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAACGTCTACAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3