Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1080871..1081491 Replicon chromosome
Accession NZ_CP010384
Organism Enterobacter hormaechei subsp. xiangfangensis strain 34399

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag LI66_RS05090 Protein ID WP_015571250.1
Coordinates 1080871..1081089 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag LI66_RS05095 Protein ID WP_006809850.1
Coordinates 1081117..1081491 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI66_RS05060 1076883..1077143 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
LI66_RS05065 1077146..1077286 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LI66_RS05070 1077283..1077993 - 711 WP_022650493.1 GNAT family N-acetyltransferase -
LI66_RS05075 1078095..1079555 + 1461 WP_022650494.1 PLP-dependent aminotransferase family protein -
LI66_RS05080 1079527..1079994 - 468 WP_022650495.1 hypothetical protein -
LI66_RS05085 1080111..1080662 - 552 WP_022650496.1 maltose O-acetyltransferase -
LI66_RS05090 1080871..1081089 - 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
LI66_RS05095 1081117..1081491 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
LI66_RS05100 1082002..1085148 - 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit -
LI66_RS05105 1085171..1086364 - 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T52301 WP_015571250.1 NZ_CP010384:c1081089-1080871 [Enterobacter hormaechei subsp. xiangfangensis]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T52301 NZ_CP010384:c1081089-1080871 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT52301 WP_006809850.1 NZ_CP010384:c1081491-1081117 [Enterobacter hormaechei subsp. xiangfangensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT52301 NZ_CP010384:c1081491-1081117 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References