Detailed information of TA system TA052300

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 581668..582371
• Replicon: chromosome
• Accession: NZ_CP010384
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain 34399

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8B5ZZ18
• Locus tag: LI66_RS02770
• Protein ID: WP_039268718.1
• Coordinates: 582030..582371 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8B5ZWQ9
• Locus tag: LI66_RS02765
• Protein ID: WP_039268717.1
• Coordinates: 581668..582009 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI66_RS02730	576821..577696	+	876	WP_039268712.1	GTPase family protein	-
LI66_RS02735	577940..578656	+	717	WP_039268713.1	WYL domain-containing protein	-
LI66_RS02740	578692..579144	+	453	WP_039269148.1	hypothetical protein	-
LI66_RS02745	579216..579689	+	474	WP_039268714.1	hypothetical protein	-
LI66_RS02750	579810..580631	+	822	WP_039268715.1	DUF945 domain-containing protein	-
LI66_RS02755	580662..581102	+	441	WP_039268716.1	antirestriction protein	-
LI66_RS02760	581115..581657	+	543	WP_045259572.1	DNA repair protein RadC	-
LI66_RS02765	581668..582009	+	342	WP_039268717.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LI66_RS02770	582030..582371	+	342	WP_039268718.1	TA system toxin CbtA family protein	Toxin
LI66_RS02775	582626..583633	+	1008	WP_039268719.1	restriction endonuclease	-
LI66_RS02780	583814..584650	-	837	Protein_534	HNH endonuclease	-
LI66_RS25415	584950..585417	+	468	WP_022650322.1	SymE family type I addiction module toxin	-
LI66_RS02790	585475..585846	-	372	WP_022650323.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	538139..590317	52178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12772.83 Da
• Isoelectric Point: 9.6548

>T52300 WP_039268718.1 NZ_CP010384:582030-582371 [Enterobacter hormaechei subsp. xiangfangensis]
MKTLPATTPQAAKLCLLPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T52300 NZ_CP010384:582030-582371 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTTGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCTTATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12729.40 Da
• Isoelectric Point: 6.9460

>AT52300 WP_039268717.1 NZ_CP010384:581668-582009 [Enterobacter hormaechei subsp. xiangfangensis]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRVSITGKFSEAECLKLDVAFPHFISQMESMLTTGGMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT52300 NZ_CP010384:581668-582009 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTAGCTGACCGGGTCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGGAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A8B5ZZ18

Antitoxin

• AlphaFold DB: A0A8B5ZWQ9