Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1705042..1705839 Replicon chromosome
Accession NZ_CP010377
Organism Enterobacter hormaechei subsp. hormaechei strain 34983

Toxin (Protein)


Gene name KacT Uniprot ID F5RT91
Locus tag LI64_RS08180 Protein ID WP_006809321.1
Coordinates 1705318..1705839 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag LI64_RS08175 Protein ID WP_001303459.1
Coordinates 1705042..1705311 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI64_RS08145 1700664..1701164 + 501 WP_032673548.1 DedA family protein -
LI64_RS08150 1701269..1702510 + 1242 WP_006809325.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
LI64_RS08155 1702543..1702770 - 228 WP_006809324.1 YccJ family protein -
LI64_RS08160 1702789..1703385 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
LI64_RS08165 1703776..1703946 + 171 WP_001273664.1 general stress protein -
LI64_RS08170 1704063..1704968 + 906 WP_039273532.1 DMT family transporter -
LI64_RS08175 1705042..1705311 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
LI64_RS08180 1705318..1705839 + 522 WP_006809321.1 GNAT family N-acetyltransferase Toxin
LI64_RS08185 1705898..1707220 - 1323 WP_006809320.1 pyrimidine utilization transport protein G -
LI64_RS08190 1707242..1707736 - 495 WP_006809319.1 pyrimidine utilization flavin reductase protein F -
LI64_RS08195 1707746..1708336 - 591 WP_006809318.1 malonic semialdehyde reductase -
LI64_RS08200 1708346..1709146 - 801 WP_006809317.1 pyrimidine utilization protein D -
LI64_RS08205 1709154..1709540 - 387 WP_006809316.1 pyrimidine utilization protein C -
LI64_RS08210 1709552..1710241 - 690 WP_006809315.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19494.28 Da        Isoelectric Point: 7.9346

>T52287 WP_006809321.1 NZ_CP010377:1705318-1705839 [Enterobacter hormaechei subsp. hormaechei]
VANLTIEMLSEGTDYDFGNFDCGEPSLNAFLTDHLVRQHSGRILRGYLLKERDHPRILGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQV

Download         Length: 522 bp

>T52287 NZ_CP010377:1705318-1705839 [Enterobacter hormaechei subsp. hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCAATTTCGATTGTGGTGAACCCTCGCT
CAATGCCTTCCTTACCGATCACCTGGTACGTCAGCACAGCGGACGTATTTTACGCGGCTACCTACTTAAAGAACGCGACC
ACCCACGTATCCTGGGCTATTACACGCTTTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGACGTCTGGCTGTCCATAAAGAACTACAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCACTGTTC
TTCCCCACGCAATCTATCGAACGACTGTTTGAGCAGGTGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT52287 WP_001303459.1 NZ_CP010377:1705042-1705311 [Enterobacter hormaechei subsp. hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT52287 NZ_CP010377:1705042-1705311 [Enterobacter hormaechei subsp. hormaechei]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTATCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGATAATCCGCCAGAGCCAAATGAACGCCTGAAACGC
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1R089

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References