Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 963608..964287 Replicon chromosome
Accession NZ_CP010377
Organism Enterobacter hormaechei subsp. hormaechei strain 34983

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2J0PMI2
Locus tag LI64_RS04600 Protein ID WP_039273191.1
Coordinates 963608..963949 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2J0PNI1
Locus tag LI64_RS04605 Protein ID WP_039273193.1
Coordinates 963970..964287 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI64_RS04580 959600..960703 + 1104 WP_006809995.1 glutamate 5-kinase -
LI64_RS04585 960715..961968 + 1254 WP_006809994.1 glutamate-5-semialdehyde dehydrogenase -
LI64_RS04595 962562..963530 + 969 WP_000654811.1 IS5 family transposase -
LI64_RS04600 963608..963949 - 342 WP_039273191.1 TA system toxin CbtA family protein Toxin
LI64_RS04605 963970..964287 - 318 WP_039273193.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LI64_RS04610 964307..964528 - 222 WP_039273195.1 DUF987 domain-containing protein -
LI64_RS04615 964537..965013 - 477 WP_039273197.1 RadC family protein -
LI64_RS04620 965029..965490 - 462 Protein_890 antirestriction protein -
LI64_RS25715 965581..965750 - 170 Protein_891 DUF905 family protein -
LI64_RS04630 966234..968231 + 1998 WP_039274705.1 choline BCCT transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 962607..974858 12251
- flank IS/Tn - - 962607..963530 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12851.82 Da        Isoelectric Point: 9.6545

>T52285 WP_039273191.1 NZ_CP010377:c963949-963608 [Enterobacter hormaechei subsp. hormaechei]
MKPLPATTSRAAKPCLSPVAVWQILLTHLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDRRGF
SRQEQSPYLRAVDILRARQVTGLLRQSRNNVVR

Download         Length: 342 bp

>T52285 NZ_CP010377:c963949-963608 [Enterobacter hormaechei subsp. hormaechei]
ATGAAACCTCTACCTGCAACAACTTCGCGGGCGGCGAAGCCTTGTCTGTCGCCCGTTGCAGTCTGGCAAATACTGCTTAC
ACACCTGCTGGAGCAGCACTATGGTCTGACGCTAAACGATACGCCGTTCAGTGATGAAACCGTTATTAAGGAACATATTG
ATGCCGGGATCACCCTGGCCGATGCCGTTAATTTTCTGGTGGAGAAATACGAGCTGGTGCGTATAGATCGCAGGGGATTT
AGCAGGCAGGAACAATCGCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGTAACTGGCCTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11890.58 Da        Isoelectric Point: 7.7498

>AT52285 WP_039273193.1 NZ_CP010377:c964287-963970 [Enterobacter hormaechei subsp. hormaechei]
MSNITWGLQRNITPRLGARLVQDRNRMHYLADRASITGKFSDAECLKLDVAFPHFISQMESMLTTGELNPRHPHCIALYH
NGFTCEADTLGSCGYVYIAVYPSQR

Download         Length: 318 bp

>AT52285 NZ_CP010377:c964287-963970 [Enterobacter hormaechei subsp. hormaechei]
ATGAGCAATATCACATGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGATAGGAACCGGAT
GCATTATCTGGCTGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAATGCCTGAAGTTGGATGTCGCATTCCCGC
ATTTTATCAGTCAGATGGAATCAATGCTGACCACCGGTGAACTGAATCCCCGCCATCCACACTGCATCGCCCTGTATCAC
AACGGTTTTACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCTGTTTATCCCAGCCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PMI2

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PNI1

References