Detailed information of TA system TA052271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2366177..2366916
• Replicon: chromosome
• Accession: NZ_CP010376
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 34977

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LI62_RS11625
• Protein ID: WP_040117655.1
• Coordinates: 2366177..2366662 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: LI62_RS11630
• Protein ID: WP_003857131.1
• Coordinates: 2366650..2366916 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI62_RS11600	2361498..2362313	-	816	WP_012695470.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-
LI62_RS11605	2362474..2363440	-	967	Protein_2230	IS5-like element IS903B family transposase	-
LI62_RS11610	2363472..2363843	-	372	Protein_2231	IS5/IS1182 family transposase	-
LI62_RS11615	2363918..2364550	+	633	WP_001567369.1	hypothetical protein	-
LI62_RS11620	2364579..2365982	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
LI62_RS11625	2366177..2366662	-	486	WP_040117655.1	GNAT family N-acetyltransferase	Toxin
LI62_RS11630	2366650..2366916	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
LI62_RS11635	2366980..2367909	-	930	WP_040117656.1	LysR family transcriptional regulator	-
LI62_RS11640	2368039..2369421	+	1383	WP_040117657.1	MFS transporter	-
LI62_RS11645	2369443..2370438	-	996	WP_040117658.1	DUF2891 domain-containing protein	-
LI62_RS11650	2370448..2371434	-	987	WP_017382341.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17540.23 Da
• Isoelectric Point: 9.9658

>T52271 WP_040117655.1 NZ_CP010376:c2366662-2366177 [Enterobacter hormaechei subsp. steigerwaltii]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIIIARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T52271 NZ_CP010376:c2366662-2366177 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGGAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTATTGCCCGTCTGGCTGTCGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGAGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTACGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT52271 WP_003857131.1 NZ_CP010376:c2366916-2366650 [Enterobacter hormaechei subsp. steigerwaltii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT52271 NZ_CP010376:c2366916-2366650 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9