Detailed information of TA system TA052270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1751118..1751915
• Replicon: chromosome
• Accession: NZ_CP010376
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 34977

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: LI62_RS08620
• Protein ID: WP_040117472.1
• Coordinates: 1751394..1751915 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: F5RT92
• Locus tag: LI62_RS08615
• Protein ID: WP_001303459.1
• Coordinates: 1751118..1751387 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI62_RS08585	1746831..1747712	+	882	WP_040117471.1	hypothetical protein	-
LI62_RS08590	1747709..1748140	+	432	WP_050597034.1	hypothetical protein	-
LI62_RS08595	1748618..1748845	-	228	WP_006809324.1	YccJ family protein	-
LI62_RS08600	1748864..1749460	-	597	WP_006809323.1	NAD(P)H:quinone oxidoreductase	-
LI62_RS08605	1749852..1750022	+	171	WP_001273664.1	general stress protein	-
LI62_RS08610	1750139..1751044	+	906	WP_017693666.1	DMT family transporter	-
LI62_RS08615	1751118..1751387	+	270	WP_001303459.1	DUF1778 domain-containing protein	Antitoxin
LI62_RS08620	1751394..1751915	+	522	WP_040117472.1	GNAT family N-acetyltransferase	Toxin
LI62_RS08625	1751974..1753296	-	1323	WP_040117473.1	pyrimidine utilization transport protein G	-
LI62_RS08630	1753318..1753809	-	492	WP_040117474.1	pyrimidine utilization flavin reductase protein F	-
LI62_RS08635	1753822..1754412	-	591	WP_040117475.1	malonic semialdehyde reductase	-
LI62_RS08640	1754422..1755222	-	801	WP_040117476.1	pyrimidine utilization protein D	-
LI62_RS08645	1755230..1755616	-	387	WP_040117477.1	pyrimidine utilization protein C	-
LI62_RS08650	1755628..1756317	-	690	WP_040117478.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19437.18 Da
• Isoelectric Point: 7.4317

>T52270 WP_040117472.1 NZ_CP010376:1751394-1751915 [Enterobacter hormaechei subsp. steigerwaltii]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTDHLVRQHGGRILRGYLLKERDHPRILGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Nucleotide

• Length: 522 bp

>T52270 NZ_CP010376:1751394-1751915 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
CAATGCCTTCCTTACCGATCACCTGGTGCGTCAGCATGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTATCCTGGGCTATTACACCCTTTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGACGTCTGGCTGTCCATAAAGAACTACAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTGGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10248.70 Da
• Isoelectric Point: 5.8836

>AT52270 WP_001303459.1 NZ_CP010376:1751118-1751387 [Enterobacter hormaechei subsp. steigerwaltii]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT52270 NZ_CP010376:1751118-1751387 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGACAATCCACCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7D7BCZ2