Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1123631..1124251 Replicon chromosome
Accession NZ_CP010376
Organism Enterobacter hormaechei subsp. steigerwaltii strain 34977

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag LI62_RS05585 Protein ID WP_015571250.1
Coordinates 1123631..1123849 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag LI62_RS05590 Protein ID WP_006809850.1
Coordinates 1123877..1124251 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI62_RS05555 1119643..1119903 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
LI62_RS05560 1119906..1120046 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LI62_RS05565 1120043..1120753 - 711 WP_023303201.1 GNAT family N-acetyltransferase -
LI62_RS05570 1120855..1122315 + 1461 WP_032647455.1 PLP-dependent aminotransferase family protein -
LI62_RS05575 1122287..1122754 - 468 WP_015571252.1 extracytoplasmic function sigma factor YlaC -
LI62_RS05580 1122871..1123422 - 552 WP_032647457.1 maltose O-acetyltransferase -
LI62_RS05585 1123631..1123849 - 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
LI62_RS05590 1123877..1124251 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
LI62_RS05595 1124762..1127908 - 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit -
LI62_RS05600 1127931..1129124 - 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T52269 WP_015571250.1 NZ_CP010376:c1123849-1123631 [Enterobacter hormaechei subsp. steigerwaltii]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T52269 NZ_CP010376:c1123849-1123631 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT52269 WP_006809850.1 NZ_CP010376:c1124251-1123877 [Enterobacter hormaechei subsp. steigerwaltii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT52269 NZ_CP010376:c1124251-1123877 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References