Detailed information of TA system TA052268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 655011..655587
• Replicon: chromosome
• Accession: NZ_CP010376
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 34977

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LI62_RS03410
• Protein ID: WP_040117186.1
• Coordinates: 655011..655298 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: LI62_RS03415
• Protein ID: WP_017694570.1
• Coordinates: 655285..655587 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI62_RS03385	650104..650823	+	720	WP_017383867.1	winged helix-turn-helix domain-containing protein	-
LI62_RS03390	650973..651443	+	471	WP_023303035.1	MarR family transcriptional regulator	-
LI62_RS03395	651440..652507	+	1068	WP_026080654.1	HlyD family secretion protein	-
LI62_RS03400	652497..653573	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
LI62_RS03405	653570..654841	-	1272	WP_015572579.1	DUF445 domain-containing protein	-
LI62_RS03410	655011..655298	+	288	WP_040117186.1	BrnT family toxin	Toxin
LI62_RS03415	655285..655587	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
LI62_RS03420	655616..656254	-	639	WP_017694569.1	LysE family translocator	-
LI62_RS03425	656293..657045	-	753	WP_015572582.1	AraC family transcriptional regulator	-
LI62_RS03430	657199..658569	+	1371	WP_040117187.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
LI62_RS03435	658745..659290	-	546	WP_006810254.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11277.76 Da
• Isoelectric Point: 6.9790

>T52268 WP_040117186.1 NZ_CP010376:655011-655298 [Enterobacter hormaechei subsp. steigerwaltii]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIHIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T52268 NZ_CP010376:655011-655298 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACATATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT52268 WP_017694570.1 NZ_CP010376:655285-655587 [Enterobacter hormaechei subsp. steigerwaltii]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT52268 NZ_CP010376:655285-655587 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA