Detailed information of TA system TA052267

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 105460..106179
• Replicon: chromosome
• Accession: NZ_CP010376
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 34977

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LI62_RS00780
• Protein ID: WP_040117033.1
• Coordinates: 105460..105780 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LI62_RS00785
• Protein ID: WP_032620726.1
• Coordinates: 105814..106179 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI62_RS00750	100675..101130	+	456	WP_040117028.1	GNAT family N-acetyltransferase	-
LI62_RS00755	101120..102157	-	1038	WP_040117029.1	virulence RhuM family protein	-
LI62_RS00760	102208..102549	-	342	WP_040117030.1	SymE family type I addiction module toxin	-
LI62_RS00765	102677..102859	+	183	WP_040117031.1	DUF3950 domain-containing protein	-
LI62_RS25010	102856..103122	+	267	WP_071887065.1	hypothetical protein	-
LI62_RS00770	103131..104372	+	1242	WP_052686654.1	DUF4935 domain-containing protein	-
LI62_RS00775	104369..105325	+	957	WP_040117032.1	hypothetical protein	-
LI62_RS00780	105460..105780	-	321	WP_040117033.1	TA system toxin CbtA family protein	Toxin
LI62_RS00785	105814..106179	-	366	WP_032620726.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LI62_RS00790	106211..106432	-	222	WP_032620724.1	DUF987 domain-containing protein	-
LI62_RS00795	106441..106911	-	471	WP_040117034.1	DNA repair protein RadC	-
LI62_RS00800	106981..107802	-	822	WP_032620720.1	DUF945 domain-containing protein	-
LI62_RS25015	108245..108436	-	192	WP_023305181.1	hypothetical protein	-
LI62_RS00805	108495..109334	-	840	WP_049125417.1	hypothetical protein	-
LI62_RS00810	109447..109881	-	435	WP_044488933.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	103131..132202	29071

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12206.77 Da
• Isoelectric Point: 5.9558

>T52267 WP_040117033.1 NZ_CP010376:c105780-105460 [Enterobacter hormaechei subsp. steigerwaltii]
MHISTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVHRAQYNLGLKRS

Nucleotide

• Length: 321 bp

>T52267 NZ_CP010376:c105780-105460 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATATTTCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGACACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTCCTTGTGGAACGATTCGAGCTTGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCTTGGATAACATCTTTGGATGTTCATCGGGCACAGTACAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13574.29 Da
• Isoelectric Point: 6.4673

>AT52267 WP_032620726.1 NZ_CP010376:c106179-105814 [Enterobacter hormaechei subsp. steigerwaltii]
MQSTTISGTSAENTSCLQWELKRNITPCFGARLVQESNRLYFLADRAGFNGSFSDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT52267 NZ_CP010376:c106179-105814 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCAGTCGACCACCATATCCGGCACATCGGCGGAGAACACATCCTGCCTTCAGTGGGAATTAAAACGTAATATCACGCC
GTGCTTCGGCGCTCGCCTGGTACAGGAAAGCAACCGCCTATACTTTCTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
GTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTGACGGCTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACACGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
CTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA