Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9898..10423 Replicon plasmid p6409-151.583kb
Accession NZ_CP010372
Organism Escherichia coli strain 6409

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag RR31_RS23630 Protein ID WP_001159871.1
Coordinates 10118..10423 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag RR31_RS23625 Protein ID WP_000813630.1
Coordinates 9898..10116 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR31_RS28445 5108..5794 + 687 Protein_3 IS1 family transposase -
RR31_RS23605 6048..7070 - 1023 WP_000361402.1 helicase UvrD -
RR31_RS23610 7055..8620 - 1566 WP_001128474.1 AAA family ATPase -
RR31_RS23615 8695..9111 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
RR31_RS23620 9108..9338 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
RR31_RS23625 9898..10116 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
RR31_RS23630 10118..10423 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
RR31_RS23635 10424..11230 + 807 WP_000016970.1 site-specific integrase -
RR31_RS23640 11952..12707 + 756 WP_000852146.1 replication initiation protein RepE -
RR31_RS23645 13295..14461 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / tet(B) / qacE / sul1 / blaTEM-1B / sitABCD iucC / iucD / iutA / iucA / iucB 1..151583 151583
- flank IS/Tn - - 5447..5794 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T52256 WP_001159871.1 NZ_CP010372:10118-10423 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T52256 NZ_CP010372:10118-10423 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT52256 WP_000813630.1 NZ_CP010372:9898-10116 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT52256 NZ_CP010372:9898-10116 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References