Detailed information of TA system TA052236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1689625..1690151
• Replicon: chromosome
• Accession: NZ_CP010371
• Organism: Escherichia coli strain 6409

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: RR31_RS08285
• Protein ID: WP_000323025.1
• Coordinates: 1689625..1689912 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: RR31_RS08290
• Protein ID: WP_000534858.1
• Coordinates: 1689912..1690151 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR31_RS08235	1684899..1685432	-	534	WP_001092971.1	lysozyme	-
RR31_RS08240	1685429..1685740	-	312	WP_000189916.1	YdfR family protein	-
RR31_RS08245	1685745..1685960	-	216	WP_000839590.1	phage lysis protein EssD	-
RR31_RS08260	1687029..1687520	-	492	WP_052424683.1	antitermination protein	-
RR31_RS08265	1687534..1688583	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
RR31_RS08270	1688585..1688863	-	279	WP_012304870.1	hypothetical protein	-
RR31_RS08275	1688930..1689181	-	252	WP_000980994.1	hypothetical protein	-
RR31_RS08280	1689398..1689553	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
RR31_RS08285	1689625..1689912	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
RR31_RS08290	1689912..1690151	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
RR31_RS26415	1690176..1690481	+	306	WP_001326990.1	hypothetical protein	-
RR31_RS08295	1690684..1691016	+	333	WP_001301033.1	protein FlxA	-
RR31_RS08300	1691453..1692766	-	1314	WP_000589005.1	ISNCY family transposase	-
RR31_RS29310	1693535..1693822	-	288	Protein_1575	hypothetical protein	-
RR31_RS08315	1694044..1694448	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
RR31_RS08320	1694445..1694792	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1653976..1705731	51755
	-	flank	IS/Tn	-	-	1686298..1686591	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T52236 WP_000323025.1 NZ_CP010371:c1689912-1689625 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T52236 NZ_CP010371:c1689912-1689625 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT52236 WP_000534858.1 NZ_CP010371:c1690151-1689912 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT52236 NZ_CP010371:c1690151-1689912 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8