Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 81988..82631 Replicon plasmid p34978-139.941kb
Accession NZ_CP010363
Organism Enterobacter hormaechei subsp. xiangfangensis strain 34978

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LI63_RS00465 Protein ID WP_001044770.1
Coordinates 81988..82404 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LI63_RS00470 Protein ID WP_001261282.1
Coordinates 82401..82631 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI63_RS26845 77879..77944 + 66 Protein_85 EAL domain-containing protein -
LI63_RS00435 78084..78848 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
LI63_RS00440 78991..79257 - 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
LI63_RS00445 79478..79951 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
LI63_RS25250 80107..80874 + 768 Protein_89 class 1 integron integrase IntI1 -
LI63_RS00455 80927..81631 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LI63_RS00460 81678..81914 - 237 WP_001348075.1 AAA family ATPase -
LI63_RS00465 81988..82404 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LI63_RS00470 82401..82631 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LI63_RS25255 82588..83048 + 461 Protein_94 hypothetical protein -
LI63_RS26850 83176..83496 + 321 WP_032072060.1 hypothetical protein -
LI63_RS00475 83545..84183 + 639 WP_022644887.1 hypothetical protein -
LI63_RS00480 84186..84971 + 786 WP_022644886.1 site-specific integrase -
LI63_RS25260 85029..85286 - 258 WP_032408936.1 hypothetical protein -
LI63_RS26855 85415..85528 - 114 WP_032072094.1 hypothetical protein -
LI63_RS00485 86159..86914 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..139941 139941


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T52224 WP_001044770.1 NZ_CP010363:c82404-81988 [Enterobacter hormaechei subsp. xiangfangensis]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T52224 NZ_CP010363:c82404-81988 [Enterobacter hormaechei subsp. xiangfangensis]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT52224 WP_001261282.1 NZ_CP010363:c82631-82401 [Enterobacter hormaechei subsp. xiangfangensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT52224 NZ_CP010363:c82631-82401 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References