Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 128614..130182 Replicon plasmid p35734-141.404kb
Accession NZ_CP010360
Organism Enterobacter roggenkampii strain 35734

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag LI67_RS00755 Protein ID WP_024134513.1
Coordinates 128875..130182 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag LI67_RS00750 Protein ID WP_039272366.1
Coordinates 128614..128865 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI67_RS00730 125364..126068 + 705 WP_016162063.1 IS6-like element IS26 family transposase -
LI67_RS00735 126380..126661 + 282 WP_000575656.1 hypothetical protein -
LI67_RS00740 126680..127243 + 564 WP_001132404.1 hypothetical protein -
LI67_RS00745 127246..128250 + 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
LI67_RS00750 128614..128865 + 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
LI67_RS00755 128875..130182 + 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
LI67_RS00760 130179..130673 + 495 WP_039272364.1 hypothetical protein -
LI67_RS00765 130848..131786 - 939 WP_044596315.1 hypothetical protein -
LI67_RS00770 131985..133625 + 1641 WP_031611419.1 PAAR domain-containing protein -
LI67_RS00780 133997..134299 - 303 WP_031611417.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..141402 141402
- flank IS/Tn - - 125364..126068 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T52223 WP_024134513.1 NZ_CP010360:128875-130182 [Enterobacter roggenkampii]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

>T52223 NZ_CP010360:128875-130182 [Enterobacter roggenkampii]
ATGCAGACGCTAACAGTGGCGATGAATGGTGAGGTCGTAGGAACACTGTACCGAGATAACAAAGGGGCCATGTCATTTCA
GTATGCCCCGGAGTGGATCTCGGAACCAGGTTCACGCGCCATTTCCATGTCACTGCCTTTGGGTCATGGCAGGATCACAG
GAAGTGAAGTCTTCAATTTCTTTAGCAACCTCCTACCCGACTCAGAGGCAATTATTGCCAGGATGCAGGCTAGGTTCAGA
GTTGAGACAACACATCCATTTGATTTGCTGGCGAGTGTTGGCCGGGATTGTGTTGGAGCTATCCAGCTCTATTCCCCCGA
TGCGGACATCCCAACTGTAATGGAGACGCTTGCAGAGCCTCTGGATGAAGCTCAGATTGAGCAACTGCTTGAAGGCTACC
AGGAAGCGCCCCTTGGGATGGCAGAGGATGCTGATTTTCGGATCTCGATTGCCGGAGCCCAAGAGAAAACTGCACTGCTT
TGGTATCAGGACCGCTGGCAACGCCCCCTGGGCAGCACCCCAACAAGCCATATTTTTAAGCTACCCATTGGTAAGATTGT
TCAAAATAACATCGATCTCAGTGAGAGTTGTGAGAATGAATGGCTCTGTTTGCGCATTGCAAAGGCCTTCGGTTTTGACG
TCGCCAATGCGGACCTAGCAACGTTCGGCAAGAAGAAGGTGCTTGTCGTTCAGCGTTTTGACCGGCGCTGGTCTAAATCC
GGCGAATGGCTTCTACGATTGCCCCAAGAAGACTTCTGCCAAGCTCTTGGGATCGCTCCTGCGCTCAAATACGAGTCACA
TGGAGGGCCAGGTATTGCTGACGCAATGAAGTTGTTGTTGGGGTCCAGACTTGCGGCTAAGGATAGAGAGCAATTTTTCA
AGTCGCAGATCCTATTCTGGATGTTGGCCGCGATTGATGGGCACGGGAAGAACTTCTCCATCTTCATTGAGCCTGCATCG
TCATTTAGGATGACCCCCCTCTACGATGTCATGTCTGCTTTTCCTATCTTCAAAAGCGGGGGAATTGCTGCTCAAAAGGC
CAAGATGGGGATGGCGCTCCAAGGCAAAAACCGCCAATACCATTTTGCGATGATCCAGCCTCGTCATTTTATCAGTACCG
CCGCCCATGTCGGGTTCCCACAAGATACCGCCGCTCAACTTATGTTCGACATGGCTACCAAGACAGAGGAGGTGGTTGCC
ACAGTAGCGGCTGAGCTGCCAGCCGGTTTCCCAGAGCACATCAGTAGCGCCATCTTCGAGGGGCTGTCCAGCCAGGCGGC
TAAGATCTTGAAGGGAGTAGCCAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT52223 WP_039272366.1 NZ_CP010360:128614-128865 [Enterobacter roggenkampii]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp

>AT52223 NZ_CP010360:128614-128865 [Enterobacter roggenkampii]
ATGAAAGTGACATCTCCATCCTTGCTTGCGGCTGCGGTGCGTGACCAGCGCAAGCTCAGCAAACTAACGCAGTCAGAGGC
GGCGAAACAGGTCGGAATCAAGCAGACGACCGTCTCTGATTTTGAACTACGGCCAGAATCTACCAAGCTGGAGACGCTAT
TCAAGCTCCTTTCTGCACTGGATCTGGAGCTTCATGTCGTGAAGCGGGGTTCAACATTGGACGATAGTAAAGTCTGGGAT
AGGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References