Detailed information of TA system TA052220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1456619..1457502
• Replicon: chromosome
• Accession: NZ_CP010359
• Organism: Pseudomonas plecoglossicida strain NyZ12

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A0C1HTG7
• Locus tag: RK21_RS06560
• Protein ID: WP_015270851.1
• Coordinates: 1457065..1457502 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: F8G488
• Locus tag: RK21_RS06555
• Protein ID: WP_013973163.1
• Coordinates: 1456619..1457068 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK21_RS06530	1451988..1453205	+	1218	WP_041505855.1	sugar transporter	-
RK21_RS06535	1453326..1453910	-	585	WP_041505856.1	LysE family translocator	-
RK21_RS06540	1453961..1454791	-	831	WP_013973166.1	AraC family transcriptional regulator	-
RK21_RS06545	1454839..1455762	-	924	WP_013973165.1	LysR family transcriptional regulator	-
RK21_RS06550	1455867..1456520	+	654	WP_013973164.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
RK21_RS06555	1456619..1457068	+	450	WP_013973163.1	DUF2384 domain-containing protein	Antitoxin
RK21_RS06560	1457065..1457502	+	438	WP_015270851.1	RES family NAD+ phosphorylase	Toxin
RK21_RS06565	1457510..1458664	-	1155	WP_013973161.1	aminotransferase class V-fold PLP-dependent enzyme	-
RK21_RS06570	1458836..1459762	-	927	WP_013973160.1	LysR family transcriptional regulator	-
RK21_RS06575	1459903..1461132	+	1230	WP_013973159.1	acyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-137)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15969.19 Da
• Isoelectric Point: 5.1199

>T52220 WP_015270851.1 NZ_CP010359:1457065-1457502 [Pseudomonas plecoglossicida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPAGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSSQTELTRGLGNRFLDECSALLLPVPSAIMPSTTNYLFNPRHPQANSARLEVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T52220 NZ_CP010359:1457065-1457502 [Pseudomonas plecoglossicida]
GTGATTCTGTGGCGTATCAGCGCCTATGCAGACTTGAGCGGAACGGGCGGCTTGCGTGTCAGCGGCCGCTGGCACCAGGC
GGGCCGGCCAGTGGTGTATGCCGCCACCAGCCCGGCCGGGGCGATGCTGGAGGTGCTGGTGCACCTGGAGATCGACCCTG
AGGATTTCCCCACCACCATGCGGCTGCTGCGTATCGAGTTGCCTGACACCGTTTCGCAAGCGCAGTTGCCCGCCTTGCAG
CCTGGCTGGTCGAGCCAGACCGAACTGACCCGGGGCCTGGGCAACCGCTTTCTGGATGAGTGTTCGGCGTTGCTGCTGCC
GGTGCCGAGCGCGATCATGCCCAGCACCACCAATTACCTTTTCAACCCGCGCCATCCGCAGGCAAACAGTGCCAGGCTCG
AGGTCGAGGACTTCACCCCGGACAGCCGGCTGTTTTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17004.59 Da
• Isoelectric Point: 6.3405

>AT52220 WP_013973163.1 NZ_CP010359:1456619-1457068 [Pseudomonas plecoglossicida]
MLAEVLRDNGYHEYRARLHALLEIPELASDFEIHTRITHGFAATWLVKLTELGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT52220 NZ_CP010359:1456619-1457068 [Pseudomonas plecoglossicida]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCATGCACTGCTGGAAATCCCCGAACT
CGCCAGTGACTTCGAAATCCACACCCGCATCACCCATGGCTTCGCTGCCACCTGGCTGGTGAAACTGACCGAACTCGGCG
TGTTCACCCCTGTGGAGCGTGACCAGATCATTCCCCTGCGCACGCTGAAAACCCGCATCGAACGTGACCAGCCGCTGACC
GTGGAAGAAAGCGACCGGCTGTTCCGTTCGGCCCATATCACCGCCATGGCCGAAGCCGTGTTCGGCGAGGCAGGCAAGGC
AAAACGCTGGCTGTCCAAGCCCAAGGAGCGCTTCTCGGGGCTGACGCCGATGCAGATGCTAACCACCCAGCAGGGCACCA
CCCAGGTCGAAGAGATGTTGCTGCAGATCGCCGAGGGCTTCGGCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0C1HTG7

Antitoxin

• AlphaFold DB: F8G488