Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 392721..393309 | Replicon | plasmid pXBB1-9 |
| Accession | NZ_CP010351 | ||
| Organism | Acinetobacter johnsonii XBB1 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | EHS42_RS19275 | Protein ID | WP_000438825.1 |
| Coordinates | 393022..393309 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | EHS42_RS19270 | Protein ID | WP_004728120.1 |
| Coordinates | 392721..393035 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EHS42_RS19230 | 388861..389133 | - | 273 | WP_000369781.1 | helix-turn-helix domain-containing protein | - |
| EHS42_RS19235 | 389126..389446 | - | 321 | WP_000897307.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EHS42_RS19240 | 389585..390004 | + | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| EHS42_RS19245 | 390084..390365 | - | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| EHS42_RS19250 | 390366..390668 | - | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EHS42_RS19255 | 390817..391002 | + | 186 | Protein_393 | hypothetical protein | - |
| EHS42_RS19260 | 391093..392225 | + | 1133 | Protein_394 | IS3-like element ISAba14 family transposase | - |
| EHS42_RS19265 | 392288..392632 | + | 345 | Protein_395 | hypothetical protein | - |
| EHS42_RS19270 | 392721..393035 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| EHS42_RS19275 | 393022..393309 | - | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| EHS42_RS19280 | 393539..394144 | - | 606 | WP_000447788.1 | LysE family translocator | - |
| EHS42_RS19285 | 394194..395021 | - | 828 | WP_000073658.1 | AraC family transcriptional regulator | - |
| EHS42_RS19290 | 395149..395901 | - | 753 | Protein_400 | IS1-like element ISAba3 family transposase | - |
| EHS42_RS19295 | 395992..396834 | - | 843 | WP_002002480.1 | carbapenem-hydrolyzing class D beta-lactamase OXA-58 | - |
| EHS42_RS19300 | 396915..397283 | + | 369 | Protein_402 | IS1-like element ISAba3 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aph(3'')-Ib / aph(6)-Id / tet(Y) / ARR-3 / qacE / sul1 / blaPER-1 / aph(3')-VIa / aac(3)-IId / cmlB1 / mph(E) / msr(E) / blaOXA-58 | - | 1..398857 | 398857 | |
| - | inside | IScluster/Tn | blaOXA-58 | - | 387433..398757 | 11324 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T52218 WP_000438825.1 NZ_CP010351:c393309-393022 [Acinetobacter johnsonii XBB1]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T52218 NZ_CP010351:c393309-393022 [Acinetobacter johnsonii XBB1]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT52218 WP_004728120.1 NZ_CP010351:c393035-392721 [Acinetobacter johnsonii XBB1]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT52218 NZ_CP010351:c393035-392721 [Acinetobacter johnsonii XBB1]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |