Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) AbkB-brnA/BrnT-BrnA
Location 379084..379672 Replicon plasmid pXBB1-9
Accession NZ_CP010351
Organism Acinetobacter johnsonii XBB1

Toxin (Protein)


Gene name AbkB Uniprot ID A0A0U3JEQ5
Locus tag EHS42_RS19175 Protein ID WP_035269327.1
Coordinates 379385..379672 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A0U3TC92
Locus tag EHS42_RS19170 Protein ID WP_032038670.1
Coordinates 379084..379398 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHS42_RS19140 374715..374951 + 237 WP_049069512.1 hypothetical protein -
EHS42_RS19145 375021..375773 - 753 WP_049069511.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
EHS42_RS19150 375777..375983 - 207 WP_049069509.1 hypothetical protein -
EHS42_RS19155 376187..376801 + 615 WP_058952609.1 AAA family ATPase -
EHS42_RS19160 376792..377817 - 1026 WP_081316866.1 IS30-like element ISAba125 family transposase -
EHS42_RS19165 377838..379028 + 1191 WP_058952610.1 AAA family ATPase -
EHS42_RS19170 379084..379398 - 315 WP_032038670.1 BrnA antitoxin family protein Antitoxin
EHS42_RS19175 379385..379672 - 288 WP_035269327.1 BrnT family toxin Toxin
EHS42_RS19180 380110..380691 + 582 WP_058952618.1 type 1 fimbrial protein -
EHS42_RS19185 380713..381423 + 711 WP_005020915.1 fimbria/pilus periplasmic chaperone -
EHS42_RS19190 381448..384009 + 2562 WP_058952611.1 fimbrial biogenesis outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / tet(Y) / ARR-3 / qacE / sul1 / blaPER-1 / aph(3')-VIa / aac(3)-IId / cmlB1 / mph(E) / msr(E) / blaOXA-58 - 1..398857 398857
- flank IS/Tn - - 376792..377802 1010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11248.64 Da        Isoelectric Point: 5.6153

>T52215 WP_035269327.1 NZ_CP010351:c379672-379385 [Acinetobacter johnsonii XBB1]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T52215 NZ_CP010351:c379672-379385 [Acinetobacter johnsonii XBB1]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCACGATGTCTCTTTCGAAACAGCAAG
CCTTGTTTTTGATGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATCGGAA
AAGTAAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACCAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12269.00 Da        Isoelectric Point: 9.4646

>AT52215 WP_032038670.1 NZ_CP010351:c379398-379084 [Acinetobacter johnsonii XBB1]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKKHQ

Download         Length: 315 bp

>AT52215 NZ_CP010351:c379398-379084 [Acinetobacter johnsonii XBB1]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACTGTTCCTGATGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ACAATCAATTCTATCGACCAGTGAAGCAACAAACATCTATTCGTTTAGATGCTGACGTACTTGCATGGTTCAAAGCACAA
GGTAAAGGTTATCAGACACGCATGAATGAAATCTTGCGGGATGCCATGCTAAAAGAATTAAAAAAACATCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0U3JEQ5

Antitoxin

Source ID Structure
AlphaFold DB A0A0U3TC92

References