Detailed information of TA system TA052199

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4511402..4511899
• Replicon: chromosome
• Accession: NZ_CP010344
• Organism: Escherichia coli ECC-1470

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: -
• Locus tag: E1470_RS22230
• Protein ID: WP_023566341.1
• Coordinates: 4511726..4511899 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: E1470_RS22225
• Protein ID: WP_071886671.1
• Coordinates: 4511402..4511698 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1470_RS22200	4508133..4508852	-	720	WP_000611288.1	two-component system response regulator DcuR	-
E1470_RS22205	4508849..4510480	-	1632	WP_001216461.1	sensor histidine kinase	-
E1470_RS22210	4510661..4510891	+	231	WP_000371700.1	(4Fe-4S)-binding protein	-
E1470_RS22215	4510903..4511175	+	273	WP_000405647.1	N-acetyltransferase	-
E1470_RS22225	4511402..4511698	+	297	WP_071886671.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
E1470_RS22230	4511726..4511899	+	174	WP_023566341.1	type V toxin-antitoxin system toxin GhoT	Toxin
E1470_RS22235	4512018..4513535	-	1518	WP_001295074.1	lysine--tRNA ligase	-
E1470_RS22240	4513772..4515229	-	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6581.10 Da
• Isoelectric Point: 9.9028

>T52199 WP_023566341.1 NZ_CP010344:4511726-4511899 [Escherichia coli ECC-1470]
MALFSKILIFYVIGVNISFVIIWFISYEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T52199 NZ_CP010344:4511726-4511899 [Escherichia coli ECC-1470]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCATA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11439.96 Da
• Isoelectric Point: 4.1705

>AT52199 WP_071886671.1 NZ_CP010344:4511402-4511698 [Escherichia coli ECC-1470]
MEGKNKFNTYVVSFDYPSSYSSVFLRLKSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT52199 NZ_CP010344:4511402-4511698 [Escherichia coli ECC-1470]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAAATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG