Detailed information of TA system TA052175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 484729..485566
• Replicon: chromosome
• Accession: NZ_CP010344
• Organism: Escherichia coli ECC-1470

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: E1470_RS02320
• Protein ID: WP_000227784.1
• Coordinates: 484729..485271 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A3T7ZCX8
• Locus tag: E1470_RS02325
• Protein ID: WP_001442339.1
• Coordinates: 485255..485566 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1470_RS02300	480268..481179	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
E1470_RS02305	481347..481838	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
E1470_RS02310	481966..483330	-	1365	WP_001000983.1	MFS transporter	-
E1470_RS02315	483738..484673	+	936	WP_001297127.1	sel1 repeat family protein	-
E1470_RS02320	484729..485271	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
E1470_RS02325	485255..485566	-	312	WP_001442339.1	DUF1778 domain-containing protein	Antitoxin
E1470_RS02330	485751..486641	-	891	WP_000971336.1	heme o synthase	-
E1470_RS02335	486653..486982	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
E1470_RS02340	486982..487596	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
E1470_RS02345	487586..489577	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
E1470_RS02350	489599..490546	-	948	WP_001239444.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T52175 WP_000227784.1 NZ_CP010344:c485271-484729 [Escherichia coli ECC-1470]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T52175 NZ_CP010344:c485271-484729 [Escherichia coli ECC-1470]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT52175 WP_001442339.1 NZ_CP010344:c485566-485255 [Escherichia coli ECC-1470]
MPGKTATLPDVDKTLKNARVEIKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT52175 NZ_CP010344:c485566-485255 [Escherichia coli ECC-1470]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAAATCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A3T7ZCX8