Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 3785713..3786380 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID O50411
Locus tag RN14_RS17765 Protein ID WP_003417916.1
Coordinates 3785713..3786105 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8U8S7
Locus tag RN14_RS17770 Protein ID WP_003912214.1
Coordinates 3786105..3786380 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS23255 (RN14_3591) 3781085..3782695 - 1611 WP_003417910.1 1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein -
RN14_RS23260 (RN14_3592) 3782640..3782921 - 282 WP_003914149.1 1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein -
RN14_RS17755 (RN14_3593) 3782918..3783907 - 990 WP_003417912.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
RN14_RS17760 (RN14_3594) 3783907..3784959 - 1053 WP_003900678.1 polyprenyl synthetase family protein -
RN14_RS17765 (RN14_3596) 3785713..3786105 - 393 WP_003417916.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS17770 (RN14_3597) 3786105..3786380 - 276 WP_003912214.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RN14_RS21620 3786562..3787933 + 1372 Protein_3530 ISNCY family transposase -
RN14_RS17785 3788123..3790209 + 2087 Protein_3531 PE family protein -
RN14_RS17790 (RN14_3602) 3790280..3791152 - 873 WP_003417929.1 3-hydroxyacyl-thioester dehydratase HtdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3787256..3787933 677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14316.74 Da        Isoelectric Point: 10.7249

>T52171 WP_003417916.1 NZ_CP010339:c3786105-3785713 [Mycobacterium tuberculosis]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Download         Length: 393 bp

>T52171 NZ_CP010339:c3786105-3785713 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9966.35 Da        Isoelectric Point: 4.5332

>AT52171 WP_003912214.1 NZ_CP010339:c3786380-3786105 [Mycobacterium tuberculosis]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Download         Length: 276 bp

>AT52171 NZ_CP010339:c3786380-3786105 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BXS8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8U8S7

References