Detailed information of TA system TA052170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3758594..3759123
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: RN14_RS17640
• Protein ID: WP_003417760.1
• Coordinates: 3758866..3759123 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: RN14_RS17635
• Protein ID: WP_003417757.1
• Coordinates: 3758594..3758869 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS23150 (RN14_3560)	3755168..3755857	-	690	WP_003917774.1	BBE domain-containing protein	-
RN14_RS23155 (RN14_3561)	3756044..3756415	-	372	WP_003417739.1	FAD-binding protein	-
RN14_RS23160	3756313..3756531	-	219	WP_157132559.1	hypothetical protein	-
RN14_RS23165 (RN14_3562)	3756558..3756818	-	261	WP_003417741.1	hypothetical protein	-
RN14_RS17620 (RN14_3563)	3756933..3757322	+	390	WP_003417745.1	DUF732 domain-containing protein	-
RN14_RS17625 (RN14_3564)	3757336..3757629	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
RN14_RS17630 (RN14_3565)	3757626..3758470	-	845	Protein_3500	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
RN14_RS17635 (RN14_3566)	3758594..3758869	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
RN14_RS17640 (RN14_3567)	3758866..3759123	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
RN14_RS17645 (RN14_3568)	3759165..3760355	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
RN14_RS17650 (RN14_3569)	3760472..3760840	+	369	WP_003417765.1	FHA domain-containing protein	-
RN14_RS17655 (RN14_3570)	3760788..3761387	-	600	WP_060811872.1	pentapeptide repeat protein MfpA	-
RN14_RS17660 (RN14_3571)	3761394..3761975	-	582	WP_003417769.1	ATP/GTP-binding protein	-
RN14_RS17665 (RN14_3572)	3761956..3762324	-	369	WP_003417772.1	DUF742 domain-containing protein	-
RN14_RS17670 (RN14_3573)	3762302..3762694	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T52170 WP_003417760.1 NZ_CP010339:3758866-3759123 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T52170 NZ_CP010339:3758866-3759123 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT52170 WP_003417757.1 NZ_CP010339:3758594-3758869 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT52170 NZ_CP010339:3758594-3758869 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94