Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3706913..3707587 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag RN14_RS17445 Protein ID WP_003417282.1
Coordinates 3706913..3707341 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag RN14_RS17450 Protein ID WP_003417286.1
Coordinates 3707345..3707587 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS17420 (RN14_3519) 3702735..3703136 - 402 WP_003417264.1 cytidine deaminase -
RN14_RS17425 (RN14_3520) 3703373..3703711 + 339 WP_003417267.1 succinate dehydrogenase, cytochrome b556 subunit -
RN14_RS17430 (RN14_3521) 3703708..3704142 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
RN14_RS17435 (RN14_3522) 3704271..3706043 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
RN14_RS17440 (RN14_3523) 3706043..3706834 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
RN14_RS17445 (RN14_3524) 3706913..3707341 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS17450 (RN14_3525) 3707345..3707587 - 243 WP_003417286.1 CopG family transcriptional regulator Antitoxin
RN14_RS17455 (RN14_3526) 3707709..3708323 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
RN14_RS17460 (RN14_3527) 3708320..3708985 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
RN14_RS17465 (RN14_3528) 3708986..3709519 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
RN14_RS17470 (RN14_3529) 3709516..3709890 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
RN14_RS17475 (RN14_3530) 3709987..3711123 - 1137 WP_003417297.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T52169 WP_003417282.1 NZ_CP010339:c3707341-3706913 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T52169 NZ_CP010339:c3707341-3706913 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT52169 WP_003417286.1 NZ_CP010339:c3707587-3707345 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT52169 NZ_CP010339:c3707587-3707345 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References