Detailed information of TA system TA052167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3181026..3181713
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: RN14_RS15060
• Protein ID: WP_003414624.1
• Coordinates: 3181270..3181713 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: RN14_RS15055
• Protein ID: WP_003414620.1
• Coordinates: 3181026..3181283 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS15035 (RN14_3041)	3176346..3177200	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
RN14_RS15040 (RN14_3042)	3177256..3178419	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
RN14_RS15045 (RN14_3043)	3178436..3179650	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
RN14_RS15050 (RN14_3044)	3179658..3180899	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
RN14_RS15055	3181026..3181283	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
RN14_RS15060 (RN14_3045)	3181270..3181713	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
RN14_RS15065 (RN14_3046)	3181793..3182455	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
RN14_RS15070 (RN14_3047)	3182551..3182742	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
RN14_RS15075 (RN14_3048)	3183074..3184819	+	1746	WP_034163967.1	cytochrome c biogenesis protein DipZ	-
RN14_RS15080 (RN14_3049)	3184915..3185496	+	582	WP_003414644.1	fasciclin domain-containing protein	-
RN14_RS15085 (RN14_3050)	3185596..3185862	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T52167 WP_003414624.1 NZ_CP010339:3181270-3181713 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T52167 NZ_CP010339:3181270-3181713 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT52167 WP_003414620.1 NZ_CP010339:3181026-3181283 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT52167 NZ_CP010339:3181026-3181283 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0