Detailed information of TA system TA052165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3134672..3135276
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: RN14_RS14830
• Protein ID: WP_003414492.1
• Coordinates: 3134672..3135064 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: RN14_RS14835
• Protein ID: WP_003414495.1
• Coordinates: 3135061..3135276 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS14800 (RN14_2994)	3129822..3130610	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
RN14_RS14805 (RN14_2995)	3130944..3131489	-	546	WP_003913758.1	DUF1802 family protein	-
RN14_RS14810 (RN14_2996)	3131761..3132645	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
RN14_RS14815 (RN14_2997)	3132648..3133535	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
RN14_RS14820 (RN14_2998)	3133840..3134385	-	546	WP_009939112.1	DUF1802 family protein	-
RN14_RS14825 (RN14_2999)	3134382..3134651	-	270	WP_003912856.1	DUF2277 family protein	-
RN14_RS14830 (RN14_3000)	3134672..3135064	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
RN14_RS14835 (RN14_3001)	3135061..3135276	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RN14_RS14840 (RN14_3002)	3135323..3136072	+	750	WP_003414497.1	enoyl-CoA hydratase	-
RN14_RS14845 (RN14_3003)	3136151..3137233	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
RN14_RS14850 (RN14_3004)	3137226..3138536	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
RN14_RS14855 (RN14_3005)	3138539..3139366	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
RN14_RS14860 (RN14_3006)	3139363..3140273	-	911	Protein_2952	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T52165 WP_003414492.1 NZ_CP010339:c3135064-3134672 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T52165 NZ_CP010339:c3135064-3134672 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT52165 WP_003414495.1 NZ_CP010339:c3135276-3135061 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT52165 NZ_CP010339:c3135276-3135061 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8