Detailed information of TA system TA052163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3108276..3108846
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: RN14_RS14680
• Protein ID: WP_003414166.1
• Coordinates: 3108276..3108632 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: RN14_RS14685
• Protein ID: WP_003901465.1
• Coordinates: 3108616..3108846 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS14660 (RN14_2966)	3103728..3105416	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
RN14_RS14665 (RN14_2967)	3105420..3105746	-	327	WP_003414157.1	hypothetical protein	-
RN14_RS14670 (RN14_2968)	3105919..3106506	+	588	WP_003914429.1	DUF3558 family protein	-
RN14_RS14675 (RN14_2969)	3106525..3108174	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
RN14_RS14680 (RN14_2970)	3108276..3108632	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
RN14_RS14685 (RN14_2971)	3108616..3108846	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
RN14_RS14690 (RN14_2972)	3108889..3109932	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
RN14_RS23090 (RN14_2973)	3109931..3110398	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
RN14_RS22295 (RN14_2974)	3110574..3110828	-	255	WP_003917684.1	hypothetical protein	-
RN14_RS14705 (RN14_2975)	3110976..3111380	+	405	WP_009938577.1	hypothetical protein	-
RN14_RS14710 (RN14_2976)	3111377..3111568	+	192	WP_003414184.1	hypothetical protein	-
RN14_RS23095	3111785..3112045	+	261	Protein_2924	transposase	-
RN14_RS14720 (RN14_2978)	3113155..3113412	+	258	WP_003899489.1	hypothetical protein	-
RN14_RS14725 (RN14_2979)	3113517..3113828	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T52163 WP_003414166.1 NZ_CP010339:c3108632-3108276 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T52163 NZ_CP010339:c3108632-3108276 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT52163 WP_003901465.1 NZ_CP010339:c3108846-3108616 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT52163 NZ_CP010339:c3108846-3108616 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3