Detailed information of TA system TA052155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2853151..2853791
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: RN14_RS13285
• Protein ID: WP_003412970.1
• Coordinates: 2853151..2853570 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: RN14_RS13290
• Protein ID: WP_003412975.1
• Coordinates: 2853567..2853791 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS13260 (RN14_2685)	2848736..2849458	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
RN14_RS13265 (RN14_2686)	2849975..2850202	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
RN14_RS13270 (RN14_2687)	2850199..2850600	+	402	WP_003412963.1	PIN domain-containing protein	-
RN14_RS13275 (RN14_2688)	2850635..2851555	-	921	WP_003412965.1	restriction endonuclease	-
RN14_RS22380	2851896..2852141	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
RN14_RS13280 (RN14_2689)	2852200..2853150	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
RN14_RS13285 (RN14_2690)	2853151..2853570	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
RN14_RS13290 (RN14_2691)	2853567..2853791	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
RN14_RS13295 (RN14_2692)	2853822..2856665	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
RN14_RS13300 (RN14_2693)	2856737..2857138	-	402	WP_003412981.1	hypothetical protein	-
RN14_RS13305 (RN14_2694)	2857138..2857608	-	471	WP_003899364.1	transcription antitermination factor NusB	-
RN14_RS13310 (RN14_2695)	2857611..2858174	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T52155 WP_003412970.1 NZ_CP010339:c2853570-2853151 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T52155 NZ_CP010339:c2853570-2853151 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT52155 WP_003412975.1 NZ_CP010339:c2853791-2853567 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT52155 NZ_CP010339:c2853791-2853567 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09