Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2806967..2807619 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag RN14_RS13085 Protein ID WP_003412752.1
Coordinates 2807194..2807619 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag RN14_RS13080 Protein ID WP_003412749.1
Coordinates 2806967..2807188 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS13065 (RN14_2650) 2805207..2805413 - 207 WP_003899347.1 hypothetical protein -
RN14_RS13070 (RN14_2651) 2805549..2806172 + 624 WP_003900858.1 TIGR00725 family protein -
RN14_RS13075 (RN14_2652) 2806162..2806914 + 753 WP_060811859.1 hypothetical protein -
RN14_RS13080 2806967..2807188 + 222 WP_003412749.1 antitoxin Antitoxin
RN14_RS13085 (RN14_2653) 2807194..2807619 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS13090 (RN14_2654) 2807642..2808823 - 1182 WP_003899351.1 dihydrolipoamide acetyltransferase family protein -
RN14_RS13095 (RN14_2655) 2808820..2809866 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
RN14_RS13100 (RN14_2656) 2809877..2810980 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
RN14_RS13105 (RN14_2657) 2811239..2812060 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
RN14_RS13110 (RN14_2658) 2812057..2812614 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T52154 WP_003412752.1 NZ_CP010339:2807194-2807619 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T52154 NZ_CP010339:2807194-2807619 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT52154 WP_003412749.1 NZ_CP010339:2806967-2807188 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT52154 NZ_CP010339:2806967-2807188 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References