Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2367147..2367842 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID O53501
Locus tag RN14_RS10985 Protein ID WP_003410811.1
Coordinates 2367147..2367581 (-) Length 145 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TMN0
Locus tag RN14_RS10990 Protein ID WP_003410814.1
Coordinates 2367588..2367842 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS10975 (RN14_2241) 2363301..2366342 + 3042 WP_031582266.1 DEAD/DEAH box helicase -
RN14_RS10980 (RN14_2242) 2366335..2367168 + 834 WP_003899172.1 SWIM zinc finger family protein -
RN14_RS10985 (RN14_2243) 2367147..2367581 - 435 WP_003410811.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS10990 (RN14_2244) 2367588..2367842 - 255 WP_003410814.1 antitoxin Antitoxin
RN14_RS10995 (RN14_2245) 2367858..2368115 - 258 WP_003410816.1 hypothetical protein -
RN14_RS11000 (RN14_2246) 2369062..2369358 + 297 WP_003410820.1 PE family protein -
RN14_RS11005 (RN14_2247) 2369414..2370145 + 732 WP_003900467.1 PPE family protein -
RN14_RS11010 (RN14_2248) 2370685..2371431 - 747 WP_003899174.1 proteasome subunit alpha -
RN14_RS11015 (RN14_2249) 2371428..2372303 - 876 WP_003411023.1 proteasome subunit beta -
RN14_RS11020 (RN14_2250) 2372300..2372494 - 195 WP_003411026.1 ubiquitin-like protein Pup -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 145 a.a.        Molecular weight: 15662.08 Da        Isoelectric Point: 7.4681

>T52152 WP_003410811.1 NZ_CP010339:c2367581-2367147 [Mycobacterium tuberculosis]
MKIVDANVLLYAVNTTSEHHKPSLRWLDGALSGADRVGFAWVPLLAFVRLATKVGLFPRPLPREAAITQVADWLAAPSAV
LVNPTVRHADILARMLTYVGTGANLVNDAHLAALAVEHRASIVSYDSDFGRFEGVRWDQPPALL

Download         Length: 435 bp

>T52152 NZ_CP010339:c2367581-2367147 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9417.57 Da        Isoelectric Point: 8.9912

>AT52152 WP_003410814.1 NZ_CP010339:c2367842-2367588 [Mycobacterium tuberculosis]
MRTTVTLDDDVEQLVRRRMAERQVSFKKALNDAIRDGASGRPAPSHFSTRTADLGVPAVNLDRALQLAADLEDEELVRRQ
RRGS

Download         Length: 255 bp

>AT52152 NZ_CP010339:c2367842-2367588 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FB09

Antitoxin

Source ID Structure
AlphaFold DB G0TMN0

References