Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2256051..2256677 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag RN14_RS10485 Protein ID WP_003410075.1
Coordinates 2256279..2256677 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag RN14_RS10480 Protein ID WP_003911750.1
Coordinates 2256051..2256278 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS10470 (RN14_2143) 2254090..2254434 - 345 WP_003410065.1 ferredoxin family protein -
RN14_RS10475 (RN14_2145) 2254623..2255792 - 1170 WP_003899126.1 ATP-binding protein -
RN14_RS10480 (RN14_2146) 2256051..2256278 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
RN14_RS10485 (RN14_2147) 2256279..2256677 + 399 WP_003410075.1 PIN domain nuclease Toxin
RN14_RS10490 (RN14_2148) 2256860..2257291 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
RN14_RS10495 (RN14_2149) 2257392..2257826 + 435 WP_003914358.1 DUF1398 domain-containing protein -
RN14_RS21180 2258263..2258442 - 180 Protein_2086 hypothetical protein -
RN14_RS10500 (RN14_2150) 2258530..2259150 + 621 WP_003410086.1 IS110 family transposase -
RN14_RS10505 (RN14_2151) 2259104..2259694 + 591 WP_003899131.1 IS110 family transposase -
RN14_RS10510 (RN14_2152) 2259822..2261077 - 1256 Protein_2089 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T52149 WP_003410075.1 NZ_CP010339:2256279-2256677 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T52149 NZ_CP010339:2256279-2256677 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT52149 WP_003911750.1 NZ_CP010339:2256051-2256278 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT52149 NZ_CP010339:2256051-2256278 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References