Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2230746..2231644 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag RN14_RS10365 Protein ID WP_003410001.1
Coordinates 2230746..2231306 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag RN14_RS10370 Protein ID WP_003410003.1
Coordinates 2231303..2231644 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS10335 (RN14_2114) 2225915..2226568 - 654 WP_003409976.1 cutinase Cfp21 -
RN14_RS10340 (RN14_2115) 2226683..2226913 + 231 WP_003409980.1 DUF167 domain-containing protein -
RN14_RS10345 (RN14_2116) 2226998..2227909 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
RN14_RS10350 (RN14_2117) 2228018..2228617 + 600 WP_003409989.1 L-lysine exporter -
RN14_RS10355 2229033..2229461 + 429 WP_003409992.1 cellulose-binding protein -
RN14_RS10360 (RN14_2119) 2229687..2230226 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
RN14_RS10365 (RN14_2120) 2230746..2231306 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
RN14_RS10370 (RN14_2121) 2231303..2231644 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
RN14_RS10375 (RN14_2122) 2231730..2231987 + 258 WP_003410006.1 hypothetical protein -
RN14_RS10380 (RN14_2123) 2231888..2232223 - 336 WP_003410009.1 dehydrogenase -
RN14_RS10385 (RN14_2124) 2232312..2232656 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
RN14_RS10390 (RN14_2125) 2232650..2232898 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
RN14_RS10395 (RN14_2126) 2232998..2235313 - 2316 WP_031582628.1 cation transporter ATPase CptG -
RN14_RS10400 (RN14_2127) 2235310..2235582 - 273 WP_003410017.1 DUF1490 family protein -
RN14_RS10405 (RN14_2128) 2235635..2235991 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T52147 WP_003410001.1 NZ_CP010339:c2231306-2230746 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T52147 NZ_CP010339:c2231306-2230746 [Mycobacterium tuberculosis]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT52147 WP_003410003.1 NZ_CP010339:c2231644-2231303 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT52147 NZ_CP010339:c2231644-2231303 [Mycobacterium tuberculosis]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References