Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2223420..2224108 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag RN14_RS10320 Protein ID WP_003409958.1
Coordinates 2223420..2223839 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag RN14_RS10325 Protein ID WP_003409968.1
Coordinates 2223848..2224108 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS10300 (RN14_2106) 2218978..2219826 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
RN14_RS10305 (RN14_2107) 2219789..2221234 - 1446 WP_003899115.1 APC family permease -
RN14_RS10310 (RN14_2108) 2221413..2222036 - 624 WP_031706292.1 RsiV family protein -
RN14_RS10315 (RN14_2109) 2222227..2223195 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
RN14_RS10320 (RN14_2111) 2223420..2223839 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS10325 (RN14_2112) 2223848..2224108 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
RN14_RS10330 (RN14_2113) 2224251..2225927 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
RN14_RS10335 (RN14_2114) 2225915..2226568 - 654 WP_003409976.1 cutinase Cfp21 -
RN14_RS10340 (RN14_2115) 2226683..2226913 + 231 WP_003409980.1 DUF167 domain-containing protein -
RN14_RS10345 (RN14_2116) 2226998..2227909 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
RN14_RS10350 (RN14_2117) 2228018..2228617 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T52146 WP_003409958.1 NZ_CP010339:c2223839-2223420 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T52146 NZ_CP010339:c2223839-2223420 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT52146 WP_003409968.1 NZ_CP010339:c2224108-2223848 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT52146 NZ_CP010339:c2224108-2223848 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References