Detailed information of TA system TA052143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2192715..2193418
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: RN14_RS10105
• Protein ID: WP_003409778.1
• Coordinates: 2192715..2193044 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: RN14_RS21155
• Protein ID: WP_003409780.1
• Coordinates: 2193041..2193418 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS10085 (RN14_2061)	2189098..2190168	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
RN14_RS10090 (RN14_2062)	2190165..2190680	+	516	WP_003409718.1	flavin reductase family protein	-
RN14_RS10095 (RN14_2063)	2190677..2191738	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
RN14_RS10100 (RN14_2064)	2191735..2192505	+	771	WP_003409775.1	SDR family oxidoreductase	-
RN14_RS10105 (RN14_2065)	2192715..2193044	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RN14_RS21155 (RN14_2066)	2193041..2193418	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
RN14_RS10115 (RN14_2067)	2193415..2194005	-	591	WP_060811853.1	SEC-C metal-binding domain-containing protein	-
RN14_RS10120	2194060..2195423	+	1364	Protein_2010	HNH endonuclease signature motif containing protein	-
RN14_RS10125	2195578..2196030	-	453	WP_003899095.1	lipoprotein	-
RN14_RS10130 (RN14_2072)	2196094..2196495	+	402	WP_003409869.1	hypothetical protein	-
RN14_RS10135	2196488..2196670	-	183	WP_003409870.1	hypothetical protein	-
RN14_RS10140 (RN14_2073)	2196784..2197134	-	351	WP_003899096.1	hypothetical protein	-
RN14_RS10145 (RN14_2074)	2197145..2198047	-	903	WP_003899097.1	hypothetical protein	-
RN14_RS10150 (RN14_2075)	2198068..2198259	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T52143 WP_003409778.1 NZ_CP010339:c2193044-2192715 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T52143 NZ_CP010339:c2193044-2192715 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT52143 WP_003409780.1 NZ_CP010339:c2193418-2193041 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT52143 NZ_CP010339:c2193418-2193041 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8