Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1939807..1940420 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag RN14_RS08995 Protein ID WP_003408465.1
Coordinates 1939807..1940196 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag RN14_RS09000 Protein ID WP_003408469.1
Coordinates 1940193..1940420 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS08960 (RN14_1823) 1935435..1936349 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
RN14_RS08965 (RN14_1824) 1936352..1937182 + 831 WP_003408448.1 cyclase family protein -
RN14_RS08970 (RN14_1825) 1937182..1937532 + 351 WP_003898986.1 cupin domain-containing protein -
RN14_RS08975 (RN14_1826) 1937585..1938404 + 820 Protein_1779 3-keto-5-aminohexanoate cleavage protein -
RN14_RS08980 (RN14_1828) 1938418..1939197 + 780 WP_003408460.1 IclR family transcriptional regulator -
RN14_RS22975 1939628..1939759 + 132 Protein_1781 IS3 family transposase -
RN14_RS08995 (RN14_1830) 1939807..1940196 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS09000 (RN14_1831) 1940193..1940420 - 228 WP_003408469.1 antitoxin Antitoxin
RN14_RS09005 (RN14_1832) 1940638..1942122 + 1485 WP_003408473.1 biotin carboxylase -
RN14_RS09010 (RN14_1833) 1942119..1943366 + 1248 WP_003408476.1 serine hydrolase -
RN14_RS09015 (RN14_1834) 1943409..1943828 - 420 WP_003408483.1 hypothetical protein -
RN14_RS09020 (RN14_1835) 1943818..1944528 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T52141 WP_003408465.1 NZ_CP010339:c1940196-1939807 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T52141 NZ_CP010339:c1940196-1939807 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT52141 WP_003408469.1 NZ_CP010339:c1940420-1940193 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT52141 NZ_CP010339:c1940420-1940193 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References