Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1688296..1688912 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag RN14_RS07900 Protein ID WP_003407593.1
Coordinates 1688595..1688912 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag RN14_RS07895 Protein ID WP_003900349.1
Coordinates 1688296..1688598 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS07885 (RN14_1603) 1684182..1686029 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
RN14_RS07890 (RN14_1604) 1686030..1688282 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
RN14_RS07895 (RN14_1605) 1688296..1688598 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
RN14_RS07900 (RN14_1606) 1688595..1688912 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
RN14_RS07905 (RN14_1607) 1688909..1689913 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
RN14_RS07910 (RN14_1608) 1689966..1691255 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
RN14_RS07915 (RN14_1609) 1691328..1692053 - 726 WP_257721563.1 class I SAM-dependent methyltransferase -
RN14_RS07920 (RN14_1610) 1692159..1692371 - 213 WP_003898900.1 dodecin family protein -
RN14_RS22960 1692432..1692830 + 399 WP_003900351.1 hypothetical protein -
RN14_RS07930 (RN14_1611) 1692875..1693903 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T52139 WP_003407593.1 NZ_CP010339:1688595-1688912 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T52139 NZ_CP010339:1688595-1688912 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT52139 WP_003900349.1 NZ_CP010339:1688296-1688598 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT52139 NZ_CP010339:1688296-1688598 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References