Detailed information of TA system TA052135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1386396..1387084
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: RN14_RS06565
• Protein ID: WP_003406304.1
• Coordinates: 1386653..1387084 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: RN14_RS06560
• Protein ID: WP_003406302.1
• Coordinates: 1386396..1386656 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS06540 (RN14_1333)	1381973..1382797	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
RN14_RS06545 (RN14_1334)	1382802..1383983	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
RN14_RS06550 (RN14_1335)	1384060..1385160	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
RN14_RS06555 (RN14_1336)	1385331..1386320	+	990	WP_003406301.1	malate dehydrogenase	-
RN14_RS06560 (RN14_1337)	1386396..1386656	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
RN14_RS06565 (RN14_1338)	1386653..1387084	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
RN14_RS06570	1387107..1388793	-	1687	Protein_1306	PE family protein	-
RN14_RS06575 (RN14_1341)	1388973..1389833	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
RN14_RS06580 (RN14_1342)	1389914..1390744	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
RN14_RS06585 (RN14_1343)	1390801..1391094	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
RN14_RS06590 (RN14_1344)	1391091..1391360	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T52135 WP_003406304.1 NZ_CP010339:1386653-1387084 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T52135 NZ_CP010339:1386653-1387084 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT52135 WP_003406302.1 NZ_CP010339:1386396-1386656 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT52135 NZ_CP010339:1386396-1386656 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6