Detailed information of TA system TA052134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241540..1242108
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: RN14_RS05900
• Protein ID: WP_003405865.1
• Coordinates: 1241734..1242108 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: RN14_RS05895
• Protein ID: WP_003405863.1
• Coordinates: 1241540..1241737 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS05875 (RN14_1194)	1237582..1238220	-	639	WP_003898730.1	lipid droplet-associated protein	-
RN14_RS05880 (RN14_1195)	1238310..1239317	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
RN14_RS05885	1239334..1240316	-	983	Protein_1167	hypothetical protein	-
RN14_RS05890 (RN14_1197)	1240379..1241452	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
RN14_RS05895 (RN14_1198)	1241540..1241737	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RN14_RS05900 (RN14_1199)	1241734..1242108	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
RN14_RS05905 (RN14_1200)	1242311..1243009	+	699	WP_003898733.1	hypothetical protein	-
RN14_RS05910 (RN14_1201)	1243127..1243312	+	186	WP_003901093.1	hypothetical protein	-
RN14_RS05915 (RN14_1202)	1243239..1243514	-	276	WP_003405867.1	hypothetical protein	-
RN14_RS05920 (RN14_1203)	1243757..1244080	+	324	WP_003405871.1	putative quinol monooxygenase	-
RN14_RS05925 (RN14_1204)	1244095..1244793	-	699	WP_031646953.1	hypothetical protein	-
RN14_RS22520 (RN14_1205)	1244827..1245036	+	210	WP_003911400.1	hypothetical protein	-
RN14_RS05935 (RN14_1207)	1244988..1245758	-	771	Protein_1177	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T52134 WP_003405865.1 NZ_CP010339:1241734-1242108 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T52134 NZ_CP010339:1241734-1242108 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT52134 WP_003405863.1 NZ_CP010339:1241540-1241737 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT52134 NZ_CP010339:1241540-1241737 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07