Detailed information of TA system TA052130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016894..1017513
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: RN14_RS04790
• Protein ID: WP_003404726.1
• Coordinates: 1017079..1017513 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: RN14_RS04785
• Protein ID: WP_003898641.1
• Coordinates: 1016894..1017073 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS04775 (RN14_0973)	1012367..1013947	+	1581	WP_003909313.1	serine hydrolase	-
RN14_RS04780 (RN14_0974)	1013944..1016337	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
RN14_RS22485 (RN14_0975)	1016610..1016768	+	159	WP_003404720.1	hypothetical protein	-
RN14_RS04785 (RN14_0976)	1016894..1017073	+	180	WP_003898641.1	antitoxin	Antitoxin
RN14_RS04790 (RN14_0977)	1017079..1017513	+	435	WP_003404726.1	SRPBCC family protein	Toxin
RN14_RS04795 (RN14_0978)	1017611..1018384	+	774	WP_003404735.1	VOC family protein	-
RN14_RS04800 (RN14_0979)	1018449..1018898	+	450	WP_003404738.1	hypothetical protein	-
RN14_RS04805 (RN14_0980)	1019033..1019263	-	231	WP_003898642.1	hypothetical protein	-
RN14_RS04810 (RN14_0981)	1019430..1020938	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
RN14_RS04815 (RN14_0982)	1020940..1022178	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T52130 WP_003404726.1 NZ_CP010339:1017079-1017513 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T52130 NZ_CP010339:1017079-1017513 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT52130 WP_003898641.1 NZ_CP010339:1016894-1017073 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT52130 NZ_CP010339:1016894-1017073 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5