Detailed information of TA system TA052128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 758908..759596
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: RN14_RS03470
• Protein ID: WP_003403386.1
• Coordinates: 758908..759345 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O06777
• Locus tag: RN14_RS03475
• Protein ID: WP_003911263.1
• Coordinates: 759342..759596 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS03440 (RN14_0706)	755159..756169	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
RN14_RS03445 (RN14_0707)	756557..756940	-	384	WP_003403365.1	ribonuclease VapC6	-
RN14_RS03450 (RN14_0708)	757035..757190	-	156	WP_031582830.1	type II toxin-antitoxin system VapB family antitoxin	-
RN14_RS03455 (RN14_0709)	757266..757982	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
RN14_RS03460 (RN14_0710)	758258..758566	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
RN14_RS03465 (RN14_0711)	758553..758798	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
RN14_RS03470 (RN14_0712)	758908..759345	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
RN14_RS03475 (RN14_0713)	759342..759596	-	255	WP_003911263.1	antitoxin VapB7	Antitoxin
RN14_RS03480 (RN14_0714)	759710..762073	+	2364	WP_031582834.1	arylsulfatase AtsD	-
RN14_RS03485 (RN14_0715)	762136..762462	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
RN14_RS03490 (RN14_0716)	762374..762712	+	339	WP_003403405.1	PIN domain-containing protein	-
RN14_RS03495	762709..762882	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T52128 WP_003403386.1 NZ_CP010339:c759345-758908 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T52128 NZ_CP010339:c759345-758908 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9450.99 Da
• Isoelectric Point: 11.7073

>AT52128 WP_003911263.1 NZ_CP010339:c759596-759342 [Mycobacterium tuberculosis]
MSMRLAHRLQILLDDECHRRITAVARERGVPVATVVREAIDRGLVSPAGRRKSAGRRLLDAADMSVPEPRELKQELEALR
ARRG

Nucleotide

• Length: 255 bp

>AT52128 NZ_CP010339:c759596-759342 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: A0A806JQG1