Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapC-mazE/PIN(toxin) |
| Location | 758553..759345 | Replicon | chromosome |
| Accession | NZ_CP010339 | ||
| Organism | Mycobacterium tuberculosis strain 22103 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P9WFB2 |
| Locus tag | RN14_RS03470 | Protein ID | WP_003403386.1 |
| Coordinates | 758908..759345 (-) | Length | 146 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G0TQE0 |
| Locus tag | RN14_RS03465 | Protein ID | WP_003403381.1 |
| Coordinates | 758553..758798 (-) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RN14_RS03435 (RN14_0704) | 753572..755078 | + | 1507 | Protein_683 | carotenoid cleavage oxygenase | - |
| RN14_RS03440 (RN14_0706) | 755159..756169 | + | 1011 | WP_003911248.1 | ABC transporter ATP-binding protein | - |
| RN14_RS03445 (RN14_0707) | 756557..756940 | - | 384 | WP_003403365.1 | ribonuclease VapC6 | - |
| RN14_RS03450 (RN14_0708) | 757035..757190 | - | 156 | WP_031582830.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| RN14_RS03455 (RN14_0709) | 757266..757982 | - | 717 | WP_003403371.1 | CPBP family intramembrane metalloprotease | - |
| RN14_RS03460 (RN14_0710) | 758258..758566 | - | 309 | WP_003403376.1 | type II toxin-antitoxin system PemK/MazF family toxin | - |
| RN14_RS03465 (RN14_0711) | 758553..758798 | - | 246 | WP_003403381.1 | ribbon-helix-helix protein, CopG family | Antitoxin |
| RN14_RS03470 (RN14_0712) | 758908..759345 | - | 438 | WP_003403386.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| RN14_RS03475 (RN14_0713) | 759342..759596 | - | 255 | WP_003911263.1 | antitoxin VapB7 | - |
| RN14_RS03480 (RN14_0714) | 759710..762073 | + | 2364 | WP_031582834.1 | arylsulfatase AtsD | - |
| RN14_RS03485 (RN14_0715) | 762136..762462 | + | 327 | WP_003403401.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| RN14_RS03490 (RN14_0716) | 762374..762712 | + | 339 | WP_003403405.1 | PIN domain-containing protein | - |
| RN14_RS03495 | 762709..762882 | + | 174 | WP_003898549.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 146 a.a. Molecular weight: 15245.39 Da Isoelectric Point: 5.1865
>T52127 WP_003403386.1 NZ_CP010339:c759345-758908 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR
Download Length: 438 bp
>T52127 NZ_CP010339:c759345-758908 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA
Antitoxin
Download Length: 82 a.a. Molecular weight: 9169.01 Da Isoelectric Point: 4.2070
>AT52127 WP_003403381.1 NZ_CP010339:c758798-758553 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R
Download Length: 246 bp
>AT52127 NZ_CP010339:c758798-758553 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U4BSW5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G0TQE0 |