Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 758258..758798 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag RN14_RS03460 Protein ID WP_003403376.1
Coordinates 758258..758566 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag RN14_RS03465 Protein ID WP_003403381.1
Coordinates 758553..758798 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS03435 (RN14_0704) 753572..755078 + 1507 Protein_683 carotenoid cleavage oxygenase -
RN14_RS03440 (RN14_0706) 755159..756169 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
RN14_RS03445 (RN14_0707) 756557..756940 - 384 WP_003403365.1 ribonuclease VapC6 -
RN14_RS03450 (RN14_0708) 757035..757190 - 156 WP_031582830.1 type II toxin-antitoxin system VapB family antitoxin -
RN14_RS03455 (RN14_0709) 757266..757982 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
RN14_RS03460 (RN14_0710) 758258..758566 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RN14_RS03465 (RN14_0711) 758553..758798 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
RN14_RS03470 (RN14_0712) 758908..759345 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
RN14_RS03475 (RN14_0713) 759342..759596 - 255 WP_003911263.1 antitoxin VapB7 -
RN14_RS03480 (RN14_0714) 759710..762073 + 2364 WP_031582834.1 arylsulfatase AtsD -
RN14_RS03485 (RN14_0715) 762136..762462 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
RN14_RS03490 (RN14_0716) 762374..762712 + 339 WP_003403405.1 PIN domain-containing protein -
RN14_RS03495 762709..762882 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T52126 WP_003403376.1 NZ_CP010339:c758566-758258 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T52126 NZ_CP010339:c758566-758258 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT52126 WP_003403381.1 NZ_CP010339:c758798-758553 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT52126 NZ_CP010339:c758798-758553 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References