Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 756557..757190 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag RN14_RS03445 Protein ID WP_003403365.1
Coordinates 756557..756940 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A655G1G4
Locus tag RN14_RS03450 Protein ID WP_031582830.1
Coordinates 757035..757190 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS03420 (RN14_0701) 751848..752384 + 537 WP_003403341.1 50S ribosomal protein L10 -
RN14_RS03425 (RN14_0702) 752421..752813 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
RN14_RS03430 (RN14_0703) 752806..753501 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
RN14_RS03435 (RN14_0704) 753572..755078 + 1507 Protein_683 carotenoid cleavage oxygenase -
RN14_RS03440 (RN14_0706) 755159..756169 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
RN14_RS03445 (RN14_0707) 756557..756940 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
RN14_RS03450 (RN14_0708) 757035..757190 - 156 WP_031582830.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
RN14_RS03455 (RN14_0709) 757266..757982 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
RN14_RS03460 (RN14_0710) 758258..758566 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
RN14_RS03465 (RN14_0711) 758553..758798 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
RN14_RS03470 (RN14_0712) 758908..759345 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
RN14_RS03475 (RN14_0713) 759342..759596 - 255 WP_003911263.1 antitoxin VapB7 -
RN14_RS03480 (RN14_0714) 759710..762073 + 2364 WP_031582834.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T52125 WP_003403365.1 NZ_CP010339:c756940-756557 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T52125 NZ_CP010339:c756940-756557 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5860.66 Da        Isoelectric Point: 5.7734

>AT52125 WP_031582830.1 NZ_CP010339:c757190-757035 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRTAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT52125 NZ_CP010339:c757190-757035 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCACCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A655G1G4

References