Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 721066..721730 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID P96917
Locus tag RN14_RS03275 Protein ID WP_003403246.1
Coordinates 721323..721730 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WF18
Locus tag RN14_RS03270 Protein ID WP_003403244.1
Coordinates 721066..721326 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS03245 (RN14_0668) 717243..718307 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
RN14_RS03250 (RN14_0669) 718411..719358 + 948 WP_003403232.1 DUF732 domain-containing protein -
RN14_RS03255 (RN14_0670) 719451..719705 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 -
RN14_RS03260 (RN14_0671) 719705..720100 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 -
RN14_RS03265 (RN14_0672) 720194..720934 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
RN14_RS03270 (RN14_0673) 721066..721326 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RN14_RS03275 (RN14_0674) 721323..721730 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS03280 (RN14_0675) 721802..722953 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
RN14_RS03285 (RN14_0676) 723046..724773 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-57)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14376.37 Da        Isoelectric Point: 4.3568

>T52124 WP_003403246.1 NZ_CP010339:721323-721730 [Mycobacterium tuberculosis]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Download         Length: 408 bp

>T52124 NZ_CP010339:721323-721730 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9492.84 Da        Isoelectric Point: 11.9108

>AT52124 WP_003403244.1 NZ_CP010339:721066-721326 [Mycobacterium tuberculosis]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT52124 NZ_CP010339:721066-721326 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3DBO

Antitoxin

Source ID Structure
PDB 3DBO
AlphaFold DB A0A7U4BSE4

References