Detailed information of TA system TA052123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 719451..720100
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67241
• Locus tag: RN14_RS03260
• Protein ID: WP_003403236.1
• Coordinates: 719705..720100 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: RN14_RS03255
• Protein ID: WP_003403235.1
• Coordinates: 719451..719705 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS22805	714577..714660	+	84	Protein_645	galactose-1-phosphate uridylyltransferase	-
RN14_RS22810	714679..715760	+	1082	Protein_646	galactose-1-phosphate uridylyltransferase	-
RN14_RS03235 (RN14_0667)	715757..716848	+	1092	WP_003906403.1	galactokinase	-
RN14_RS03245 (RN14_0668)	717243..718307	+	1065	WP_003898532.1	zinc ribbon domain-containing protein	-
RN14_RS03250 (RN14_0669)	718411..719358	+	948	WP_003403232.1	DUF732 domain-containing protein	-
RN14_RS03255 (RN14_0670)	719451..719705	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
RN14_RS03260 (RN14_0671)	719705..720100	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
RN14_RS03265 (RN14_0672)	720194..720934	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
RN14_RS03270 (RN14_0673)	721066..721326	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
RN14_RS03275 (RN14_0674)	721323..721730	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
RN14_RS03280 (RN14_0675)	721802..722953	-	1152	WP_003403248.1	FIST N-terminal domain-containing protein	-
RN14_RS03285 (RN14_0676)	723046..724773	-	1728	WP_003900979.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14250.04 Da
• Isoelectric Point: 4.7475

>T52123 WP_003403236.1 NZ_CP010339:719705-720100 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T52123 NZ_CP010339:719705-720100 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT52123 WP_003403235.1 NZ_CP010339:719451-719705 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT52123 NZ_CP010339:719451-719705 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• PDB: 4XGR
• PDB: 4XGQ

Antitoxin

• AlphaFold DB: A0A7U4BSC2