Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 719451..720100 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag RN14_RS03260 Protein ID WP_003403236.1
Coordinates 719705..720100 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag RN14_RS03255 Protein ID WP_003403235.1
Coordinates 719451..719705 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS22805 714577..714660 + 84 Protein_645 galactose-1-phosphate uridylyltransferase -
RN14_RS22810 714679..715760 + 1082 Protein_646 galactose-1-phosphate uridylyltransferase -
RN14_RS03235 (RN14_0667) 715757..716848 + 1092 WP_003906403.1 galactokinase -
RN14_RS03245 (RN14_0668) 717243..718307 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
RN14_RS03250 (RN14_0669) 718411..719358 + 948 WP_003403232.1 DUF732 domain-containing protein -
RN14_RS03255 (RN14_0670) 719451..719705 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
RN14_RS03260 (RN14_0671) 719705..720100 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
RN14_RS03265 (RN14_0672) 720194..720934 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
RN14_RS03270 (RN14_0673) 721066..721326 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
RN14_RS03275 (RN14_0674) 721323..721730 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
RN14_RS03280 (RN14_0675) 721802..722953 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
RN14_RS03285 (RN14_0676) 723046..724773 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T52123 WP_003403236.1 NZ_CP010339:719705-720100 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T52123 NZ_CP010339:719705-720100 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT52123 WP_003403235.1 NZ_CP010339:719451-719705 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT52123 NZ_CP010339:719451-719705 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References