Detailed information of TA system TA052120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 700195..700841
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: RN14_RS03115
• Protein ID: WP_003403137.1
• Coordinates: 700195..700608 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: RN14_RS03120
• Protein ID: WP_003403139.1
• Coordinates: 700605..700841 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS03095 (RN14_0639)	696278..697828	+	1551	WP_003403119.1	MlaD family protein	-
RN14_RS03100 (RN14_0640)	697880..698272	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
RN14_RS03105 (RN14_0641)	698269..698526	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
RN14_RS03110 (RN14_0642)	698709..699944	-	1236	WP_003403128.1	ATP-binding protein	-
RN14_RS03115 (RN14_0644)	700195..700608	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
RN14_RS03120 (RN14_0645)	700605..700841	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
RN14_RS03125 (RN14_0646)	700945..701451	-	507	WP_003900973.1	two component system sensor kinase HK1	-
RN14_RS03130 (RN14_0647)	701565..702095	-	531	WP_003917320.1	two component system sensor kinase HK2	-
RN14_RS03135 (RN14_0648)	702079..702774	-	696	WP_003918099.1	two-component system response regulator TcrA	-
RN14_RS03140 (RN14_0649)	702897..703208	+	312	WP_003403164.1	hypothetical protein	-
RN14_RS03145 (RN14_0650)	703280..704230	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
RN14_RS03150 (RN14_0651)	704471..705055	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
RN14_RS03155 (RN14_0652)	705057..705767	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T52120 WP_003403137.1 NZ_CP010339:c700608-700195 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T52120 NZ_CP010339:c700608-700195 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT52120 WP_003403139.1 NZ_CP010339:c700841-700605 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT52120 NZ_CP010339:c700841-700605 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89