Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 697880..698526 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag RN14_RS03100 Protein ID WP_003403122.1
Coordinates 697880..698272 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag RN14_RS03105 Protein ID WP_003403125.1
Coordinates 698269..698526 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS03085 (RN14_0637) 693542..695068 + 1527 WP_003403112.1 virulence factor Mce family protein -
RN14_RS03090 (RN14_0638) 695131..696273 + 1143 WP_003911253.1 virulence factor Mce family protein -
RN14_RS03095 (RN14_0639) 696278..697828 + 1551 WP_003403119.1 MlaD family protein -
RN14_RS03100 (RN14_0640) 697880..698272 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
RN14_RS03105 (RN14_0641) 698269..698526 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
RN14_RS03110 (RN14_0642) 698709..699944 - 1236 WP_003403128.1 ATP-binding protein -
RN14_RS03115 (RN14_0644) 700195..700608 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
RN14_RS03120 (RN14_0645) 700605..700841 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
RN14_RS03125 (RN14_0646) 700945..701451 - 507 WP_003900973.1 two component system sensor kinase HK1 -
RN14_RS03130 (RN14_0647) 701565..702095 - 531 WP_003917320.1 two component system sensor kinase HK2 -
RN14_RS03135 (RN14_0648) 702079..702774 - 696 WP_003918099.1 two-component system response regulator TcrA -
RN14_RS03140 (RN14_0649) 702897..703208 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T52119 WP_003403122.1 NZ_CP010339:c698272-697880 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T52119 NZ_CP010339:c698272-697880 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT52119 WP_003403125.1 NZ_CP010339:c698526-698269 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT52119 NZ_CP010339:c698526-698269 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References