52117

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 643271..643947 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag RN14_RS02865 Protein ID WP_003898500.1
Coordinates 643271..643684 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag RN14_RS02870 Protein ID WP_003402915.1
Coordinates 643681..643947 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS02835 (RN14_0582) 638616..638918 - 303 WP_003402896.1 DUF3349 domain-containing protein -
RN14_RS02840 (RN14_0583) 638978..639256 - 279 WP_031706351.1 hypothetical protein -
RN14_RS02845 (RN14_0584) 639253..640506 - 1254 WP_003402904.1 inorganic phosphate transporter -
RN14_RS02850 (RN14_0585) 640626..641012 - 387 WP_003402907.1 VOC family protein -
RN14_RS02855 (RN14_0586) 641075..641959 - 885 WP_003402909.1 SDR family oxidoreductase -
RN14_RS02860 (RN14_0587) 642055..642957 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
RN14_RS02865 (RN14_0588) 643271..643684 - 414 WP_003898500.1 type II toxin-antitoxin system VapC family toxin Toxin
RN14_RS02870 (RN14_0589) 643681..643947 - 267 WP_003402915.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
RN14_RS02875 (RN14_0590) 644139..645854 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
RN14_RS02880 (RN14_0591) 645932..647536 + 1605 WP_003402920.1 amidohydrolase -
RN14_RS02885 (RN14_0592) 647533..648513 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T52117 WP_003898500.1 NZ_CP010339:c643684-643271 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T52117 NZ_CP010339:c643684-643271 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT52117 WP_003402915.1 NZ_CP010339:c643947-643681 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT52117 NZ_CP010339:c643947-643681 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References