Detailed information of TA system TA052114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366448..367019
• Replicon: chromosome
• Accession: NZ_CP010339
• Organism: Mycobacterium tuberculosis strain 22103

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: RN14_RS01580
• Protein ID: WP_003401560.1
• Coordinates: 366448..366750 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: RN14_RS01585
• Protein ID: WP_003401563.1
• Coordinates: 366798..367019 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN14_RS01560 (RN14_0325)	361917..362720	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
RN14_RS01565 (RN14_0326)	362730..364127	-	1398	WP_003401544.1	sulfatase	-
RN14_RS01570 (RN14_0327)	364306..366081	+	1776	WP_010886074.1	PE family protein	-
RN14_RS01575 (RN14_0328)	366224..366451	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
RN14_RS01580 (RN14_0329)	366448..366750	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
RN14_RS01585 (RN14_0330)	366798..367019	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
RN14_RS01590 (RN14_0331)	367016..367441	+	426	WP_003401566.1	PIN domain nuclease	-
RN14_RS01595 (RN14_0332)	367576..368208	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
RN14_RS01600 (RN14_0333)	368205..369113	+	909	WP_003900117.1	protochlorophyllide reductase	-
RN14_RS22770	369121..369711	-	591	WP_229298012.1	hypothetical protein	-
RN14_RS22775	369704..369754	-	51	Protein_320	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T52114 WP_003401560.1 NZ_CP010339:366448-366750 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T52114 NZ_CP010339:366448-366750 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT52114 WP_003401563.1 NZ_CP010339:366798-367019 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT52114 NZ_CP010339:366798-367019 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48